Campbell Biology in Focus (2nd Edition)
2nd Edition
ISBN: 9780321962751
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece
Publisher: PEARSON
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Textbook Question
Chapter 15.1, Problem 2CC
Describe the binding of RNA polymerase, repressors, and activators to the lac operon when both lactose and glucose are scarce. What is the effect of these scarcities on transcription of the lac operon?
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Describe the binding of RNA polymerase, repressors, andactivators to the lac operon when both lactose and glucose are scarce. What is the effect of these scarcities ontranscription of the lac operon?
Explain why inactivation of the O1 sequence of the lac operator almost completely abolishes repression of the lac operon.
Describe the mechanism by which cAMP receptorprotein (CRP), the regulatory protein for catabolite repression, functions. Use the lactose operon as an example.
Chapter 15 Solutions
Campbell Biology in Focus (2nd Edition)
Ch. 15.1 - How does binding of the trp corepressor to its...Ch. 15.1 - Describe the binding of RNA polymerase,...Ch. 15.1 - WHAT IF? A certain mutation in E. coli changes the...Ch. 15.2 - Prob. 1CCCh. 15.2 - Compare the roles of general and specific...Ch. 15.2 - Prob. 3CCCh. 15.3 - WHAT IF? Suppose the mRNA being degraded in Figure...Ch. 15.3 - MAKE CONNECTIONS Inactivation of one of the X...Ch. 15.4 - Describe the role of complementary base pairing...Ch. 15.4 - WHAT IF? Study the microarray in Figure 15.17. If...
Ch. 15 - If a particular operon encodes enzymes for making...Ch. 15 - The functioning of enhancers is an example of A. a...Ch. 15 - Which of the following is an example of...Ch. 15 - Prob. 4TYUCh. 15 - Prob. 5TYUCh. 15 - Which of the following would not be true of cDNA...Ch. 15 - Prob. 7TYUCh. 15 - SCIENTIFIC INQUIRY Imagine you want to study one...Ch. 15 - FOCUS ON EVOLUTION DNA sequences can act as tape...Ch. 15 - FOCUS ON INTERACTIONS In a short essay (100150...Ch. 15 - Prob. 11TYU
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- The lac operon consists of regulatory regions such as the promoter as well as the structural genes lacZ, lacY, and lacA, which code for proteins involved in lactose metabolism. What would be the outcome of a mutation in lacA?arrow_forwardWhat would happen if the operator sequence of the lac operon contained a mutation that prevented the repressor protein from binding the operator? (Explain what would happen both in the presence and absence of lactose)arrow_forwardDescribe the role of the CRP–cAMP complex in positiveregulation of the lactose operon in E. coli.arrow_forward
- Describe the probable genetic defect that abolishes the sensitivity of the lac operon to the absence of glucose when other metabolic operons continue to be sensitive to the absence of glucose.arrow_forwardExplain the role of CAP and cAMP in the regulation of the lac-operon.arrow_forwardMutation in the regulatory gene in the lac operon results in the formation of an inactive repressor. List and explain the effect of such a mutation on the expression of the structural gene for lactose in E.coli?arrow_forward
- A number of mutations affect the expression of the lac operon in E. coli. Consider each genotype below and complete the table using “+” to indicate that the gene is expressed, and “−” to indicate that gene is not expressed.arrow_forwardWhy it is essential to the positive stimulation of transcription of the lac operon ?arrow_forwardOne way that regulation is often studied is by examining the effects of mutations. For example, mutations within the parts of the lac operon have been well studied. Which of these mutants would be considered a constitutive mutant (the operon is always on)? a mutation in CAP that prevents the binding of CAMP a mutation in one of the structural genes that prevents the transport of lactose into the cell a mutation that disables the promoter for the operon a mutation that disables the repressorarrow_forward
- Supply the words “off" or “on" in the blanks below: In an inducible operon, transcription is normally and must be turned In a repressible operon, transcription is normally and must be turned Briefly describe the overall function of the structural genes in the lac operon. Briefly describe the overall function of the structural genes in the trp operon. For the following descriptions of the bacterial lac operon, determine if lactose is present (P) or absent (A). The repressor is bound to the operator. The repressor is inactive. RNA polymerase transcribes the structural genes. Allolactose is bound to the repressor For the following descriptions of the bacterial trp operon, determine if tryptophan levels are high (H) or low (L). The repressor is bound to the operator. The repressor is inactive. RNA polymerase transcribes the structural genes. Tryptophan is bound to the repressor.arrow_forwardIn the lac operon (below), how will expression of the genes lacZ and lacy be effect by a mutation in the repressor protein (encoded by lach) that prevents it from binding the operator sequence (lacO) in the absence of lactose? Explain the answerarrow_forwardThe L-arabinose operon, also called ara, is an operon required to break down the sugar L-arabinose in E. coli. This operon contains 3 structural genes in the following order, araB, araA, araD and expression is controlled by the product of a repressor called araC and the CAP protein which is regulated by cAMP in the same way as it is for the lactose operon. Now Draw a schematic of what this operon might look like when glucose levels are low and arabinose levels are high. Include the relevant proteins and their effector molecules, the promoter, operator, and structural genes. Note if the operon is being transcribed or not.arrow_forward
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