EBK BIOLOGY
4th Edition
ISBN: 8220102797376
Author: BROOKER
Publisher: YUZU
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Chapter 15.1, Problem 1BC
Summary Introduction
To describe: The role of checkpoint proteins in the prevention of cancer.
Introduction: Checkpoints help in regulating the process of the eukaryotic cell cycle. Checkpoint proteins synthesized at different checkpoints present in the eukaryotic cell cycle. These checkpoint proteins help in the prevention of cancer. Checkpoints are located at G1, G2 and M phases of eukaryotic cell cycle.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 15 Solutions
EBK BIOLOGY
Ch. 15.1 - Researchers usually treat cells with drugs that...Ch. 15.1 - Which phases make up interphase?Ch. 15.1 - Prob. 1BCCh. 15.1 - Prob. 1EQCh. 15.1 - The Eukaryotic Cell Cycle CoreSKILL What...Ch. 15.1 - Prob. 3EQCh. 15.2 - Prob. 1CCCh. 15.2 - Prob. 2CCCh. 15.2 - Mitotic Cell Division Concept Check: What are the...Ch. 15.3 - Prob. 1CC
Ch. 15.3 - Sexual Reproduction Concept Check: What is the...Ch. 15.4 - Variation in Chromosome Structure and Number...Ch. 15 - Prob. 1TYCh. 15 - Prob. 2TYCh. 15 - Prob. 3TYCh. 15 - Prob. 4TYCh. 15 - Prob. 5TYCh. 15 - Which of the following is not an event of anaphase...Ch. 15 - Prob. 7TYCh. 15 - Which of the following statements accurately...Ch. 15 - Prob. 9TYCh. 15 - Aneuploidy may be the result of a. duplication of...Ch. 15 - Prob. 1CQCh. 15 - Prob. 2CQCh. 15 - Prob. 3CQCh. 15 - Prob. 1COQCh. 15 - A diploid eukaryotic cell has 10 chromosomes (5...
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