Campbell Biology In Focus, Loose-leaf Edition (3rd Edition)
3rd Edition
ISBN: 9780134895727
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 15, Problem 6TYU
Which of the following would not be true of cDNA produced using human brain tissue as the starting material?
- A. It could be amplified by the polymerase chain reaction.
- B. It was produced from pre-mRNA using reverse transcriptase.
- C. It could be labeled and used as a probe to detect genes expressed in the brain.
- D. It lacks the introns of the pre-mRNA.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
What is the function of the sigma subunit of prokaryotic RNA polymerase?
a. It directs the polymerase to bind a specific type of promoter sequence
b. It contains the polymerase active site.
c. It allows template-independent RNA synthesis
d. It signals the polymerase to terminate transcription at specific sites.
e. It contains the separate exonuclease site required for proofreading.
Which of these statements is correct regarding the CRISPR/Cas9 system in eukaryotic cells?
A.Single guide RNA (sgRNA) is used to target specific DNA sequences and activate Cas.
B.Bacteriophages are used to inject viral genes into the cell to activated the Cas protein.
C.CRISPR takes advantage of the cell’s DNA polymerase proof-reading activities for DNA replication errors
D.The CRISPR-Cas system can only be used to alter genomes in prokaryotic cells.
E. Cas cuts directly into palindromic restriction sites that it targets in the DNA.
Which of these statements is correct regarding the CRISPR/Cas9 system in eukaryotic cells?
a.Single guide RNA (sgRNA) is used to target specific DNA sequences and activate Cas.
b.Bacteriophages are used to inject viral genes into the cell to activated the Cas protein.
c.CRISPR takes advantage of the cell’s DNA polymerase proof-reading activities for DNA replication errors
d.The CRISPR-Cas system can only be used to alter genomes in prokaryotic cells.
e.Cas cuts directly into palindromic restriction sites that it targets in the DNA.
Chapter 15 Solutions
Campbell Biology In Focus, Loose-leaf Edition (3rd Edition)
Ch. 15.1 - How does binding of the trp corepressor to its...Ch. 15.1 - Describe the binding of RNA polymerase,...Ch. 15.1 - WHAT IF? A certain mutation in E. coli changes the...Ch. 15.2 - Prob. 1CCCh. 15.2 - Compare the roles of general and specific...Ch. 15.2 - Prob. 3CCCh. 15.3 - WHAT IF? Suppose the mRNA being degraded in Figure...Ch. 15.3 - MAKE CONNECTIONS Inactivation of one of the X...Ch. 15.4 - Prob. 1CCCh. 15.4 - WHAT IF? Study the microarray in Figure 15.17. If...
Ch. 15 - If a particular operon encodes enzymes for making...Ch. 15 - The functioning of enhancers is an example of A. a...Ch. 15 - Which of the following is an example of...Ch. 15 - Prob. 4TYUCh. 15 - Prob. 5TYUCh. 15 - Which of the following would not be true of cDNA...Ch. 15 - Prob. 7TYUCh. 15 - SCIENTIFIC INQUIRY Imagine you want to study one...Ch. 15 - FOCUS ON EVOLUTION DNA sequences can act as tape...Ch. 15 - FOCUS ON INTERACTIONS In a short essay (100150...Ch. 15 - Prob. 11TYU
Additional Science Textbook Solutions
Find more solutions based on key concepts
How does the removal of hydrogen atoms from nutrient molecules result in a loss of energy from the nutrient mol...
SEELEY'S ANATOMY+PHYSIOLOGY
True or false? Some trails are considered vestigial because they existed long ago.
Biological Science (6th Edition)
An obese 55-year-old woman consults her physician about minor chest pains during exercise. Explain the physicia...
Biology: Life on Earth with Physiology (11th Edition)
Single penny tossed 20 times and counting heads and tails: Probability (prediction): _______/20 heads ________/...
Laboratory Manual For Human Anatomy & Physiology
To test your knowledge, discuss the following topics with a study partner or in writing ideally from memory. Th...
HUMAN ANATOMY
Gregor Mendel never saw a gene, yet he concluded that some inherited factors were responsible for the patterns ...
Campbell Essential Biology (7th Edition)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What are two reasons that a eukaryotic gene (e.g. Green Fluorescent Protein in jellyfish) will not be expressed if it is inserted into a bacterial genome with no alterations? a. Bacterial genes do not contain introns b. Bacterial promoters have different consensus sequences than eukaryotic ones c. Transcription of this gene is affected by the simultaneous processes of transcription and translation d.arrow_forwardSomeone submits a saliva sample for a PCR test to determine whether he is infected withSARS-CoV-2, an RNA virus. The first step is to purify RNA from the sample. Next, the RNA isconverted into cDNA.A. Why is this conversion important B. What is the first enzyme required for this step? C. Does this enzyme require a primer? Briefly explain. D. Three days later, the person submits another sample. The qPCR data againindicates a positive result; however, the spike gene Ct value is now 33. Based on the PCRdata, are they at the beginning or end of a COVID viral infection? Explain how you made thisdeterminationarrow_forwardDesign a strategy to make a subtracted-complete cDNA library from liver cells. Use a flowchart method to propose how this objective can be accomplished. The sizes of mRNA range from 2 kb to 15 kb. Briefly indicate your reasons for the sequence of steps..arrow_forward
- What strategy does a genetically encoded calcium indicator look like to allow fluorescence imaging of only one cell type in an acute slice of the brain? A.The use of fluorescent protein expression inhibitors in other cells B.The injection of a recombinant virus causing the death of other cells C.The use of a promoter specific to these cells D.Activation of membrane receptors specific to these cellsarrow_forwardA mouse gene was identified and determined to be required for formation of heart muscle. A gene with a similar sequence was identified in the human genome. What experiment could scientists do to determine if the mouse and human genes have similar functions? A. The scientist could place the normal human gene into normal mice and see if the resulting mice are viable. B. The scientist could search the human genome for genes that encode proteins that are identical to the protein encoded by the mouse gene. C. The scientist could place the normal human gene into mutant mice to see if heart muscle forms in the mouse. D. The scientist could place the mutant mouse gene into humans to see if humans develop without heart muscle.arrow_forwardArrange the steps that would be used in a laboratory to engineer a bacterium that could express the human gene coding for factor VIII. (Not all steps will be placed).arrow_forward
- Site-directed mutagenesis can be used to: a. treat diseases by gene therapy. b. make single residue mutation of a protein. c. make human insulin for treatment of diabetes. d. determine protein structures. e. determine DNA sequencingarrow_forwardE. coli are grown on a medium containing lactose. Once glucose is added to the medium, the bacteria stop fermenting lactose. Which of the following BEST explains the observed effect? A. Cellular levels of cAMP are lowB.Glucose is bound to the promoter C. Repressor protein is bound to the operator D. Repressor protein is bound to the promoterarrow_forwardThe polymerase chain reaction requires the following except A. primers complementary to the ends of the sequence to be amplified B. carefully controlled temperature conditions C. cycles of denaturation, annealing, and extension D. RNA polymerase to synthesize RNA primersarrow_forward
- David Pribnow studied the base sequences of promoters in bacteria and bacterial viruses. He found two conserved regions in these promoters (the -10 box and the -35 box). These two regions of the promoter Select one: a. signal the initiation site b. bind the sigma subunit that is associated with RNA polymerase O c. attach the correct nucleotide triphosphate to the template DNA strand d. separate the two DNA strandsarrow_forwardWhich of the following is true? a. Enhancers are proteins that bind to activator DNA sequences. b. Activators are proteins that bind to insulator sequences c. Repressors are proteins that bind to enhancer DNA sequences. d. Repressors are proteins which bind to silencer DNA sequencers.arrow_forwardCDNA libraries Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a a) contain only those pieces of DNA that are transcribed. b) contain all the DNA fragments from an organism C. c) both a and b d. none of the abovearrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
QCE Biology: Introduction to Gene Expression; Author: Atomi;https://www.youtube.com/watch?v=a7hydUtCIJk;License: Standard YouTube License, CC-BY