Campbell Biology In Focus, Loose-leaf Edition (3rd Edition)
3rd Edition
ISBN: 9780134895727
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky
Publisher: PEARSON
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Textbook Question
Chapter 15, Problem 6TYU
Which of the following would not be true of cDNA produced using human brain tissue as the starting material?
- A. It could be amplified by the polymerase chain reaction.
- B. It was produced from pre-mRNA using reverse transcriptase.
- C. It could be labeled and used as a probe to detect genes expressed in the brain.
- D. It lacks the introns of the pre-mRNA.
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What is the function of the sigma subunit of prokaryotic RNA polymerase?
a. It directs the polymerase to bind a specific type of promoter sequence
b. It contains the polymerase active site.
c. It allows template-independent RNA synthesis
d. It signals the polymerase to terminate transcription at specific sites.
e. It contains the separate exonuclease site required for proofreading.
Which of these statements is correct regarding the CRISPR/Cas9 system in eukaryotic cells?
A.Single guide RNA (sgRNA) is used to target specific DNA sequences and activate Cas.
B.Bacteriophages are used to inject viral genes into the cell to activated the Cas protein.
C.CRISPR takes advantage of the cell’s DNA polymerase proof-reading activities for DNA replication errors
D.The CRISPR-Cas system can only be used to alter genomes in prokaryotic cells.
E. Cas cuts directly into palindromic restriction sites that it targets in the DNA.
Which of these statements is correct regarding the CRISPR/Cas9 system in eukaryotic cells?
a.Single guide RNA (sgRNA) is used to target specific DNA sequences and activate Cas.
b.Bacteriophages are used to inject viral genes into the cell to activated the Cas protein.
c.CRISPR takes advantage of the cell’s DNA polymerase proof-reading activities for DNA replication errors
d.The CRISPR-Cas system can only be used to alter genomes in prokaryotic cells.
e.Cas cuts directly into palindromic restriction sites that it targets in the DNA.
Chapter 15 Solutions
Campbell Biology In Focus, Loose-leaf Edition (3rd Edition)
Ch. 15.1 - How does binding of the trp corepressor to its...Ch. 15.1 - Describe the binding of RNA polymerase,...Ch. 15.1 - WHAT IF? A certain mutation in E. coli changes the...Ch. 15.2 - Prob. 1CCCh. 15.2 - Compare the roles of general and specific...Ch. 15.2 - Prob. 3CCCh. 15.3 - WHAT IF? Suppose the mRNA being degraded in Figure...Ch. 15.3 - MAKE CONNECTIONS Inactivation of one of the X...Ch. 15.4 - Prob. 1CCCh. 15.4 - WHAT IF? Study the microarray in Figure 15.17. If...
Ch. 15 - If a particular operon encodes enzymes for making...Ch. 15 - The functioning of enhancers is an example of A. a...Ch. 15 - Which of the following is an example of...Ch. 15 - Prob. 4TYUCh. 15 - Prob. 5TYUCh. 15 - Which of the following would not be true of cDNA...Ch. 15 - Prob. 7TYUCh. 15 - SCIENTIFIC INQUIRY Imagine you want to study one...Ch. 15 - FOCUS ON EVOLUTION DNA sequences can act as tape...Ch. 15 - FOCUS ON INTERACTIONS In a short essay (100150...Ch. 15 - Prob. 11TYU
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- Match the activity below with the correct enzyme. (You won't use all the enzymes listed.) RNA acts as a template for DNA synthesis: RNA directs the cutting of an RNA molecule at a precise location: RNA directs the cutting of an DNA molecule at a precise location: options: a. Small Nuclear Ribonuclear Protein (SNRNP) b. telomerase c. primase d. helicase e. CRISPR/Cas9arrow_forwardWhich of the following is not true of cDNA produced using human brain tissue as the starting material? (A) It can be amplified by the polymerase chain reaction. (B) It was produced from pre-mRNA using reverse transcriptase. (C) It can be labeled and used as a probe to detect genes expressed in the brain. (D) It lacks the introns of the premRNAarrow_forwardA geneticist induces a mutation in eukaryote cells. The mutation results in an inability to form the poly(A) tail during processing of pre-mRNA. What does this mean for the mature mRNA and what will be the effect on these cells? Possible Answers: A. The mRNA will be spliced, but will not have a 5' cap. B. The mRNA will likely be degraded. C. The mRNA will not be cleaved. D. The mRNA will have too many Gs and Cs.arrow_forward
- A mouse gene was identified and determined to be required for formation of heart muscle. A gene with a similar sequence was identified in the human genome. What experiment could scientists do to determine if the mouse and human genes have similar functions? A. The scientist could place the normal human gene into normal mice and see if the resulting mice are viable. B. The scientist could search the human genome for genes that encode proteins that are identical to the protein encoded by the mouse gene. C. The scientist could place the normal human gene into mutant mice to see if heart muscle forms in the mouse. D. The scientist could place the mutant mouse gene into humans to see if humans develop without heart muscle.arrow_forwardArrange the steps that would be used in a laboratory to engineer a bacterium that could express the human gene coding for factor VIII. (Not all steps will be placed).arrow_forwardSite-directed mutagenesis can be used to: a. treat diseases by gene therapy. b. make single residue mutation of a protein. c. make human insulin for treatment of diabetes. d. determine protein structures. e. determine DNA sequencingarrow_forward
- The polymerase chain reaction requires the following except A. primers complementary to the ends of the sequence to be amplified B. carefully controlled temperature conditions C. cycles of denaturation, annealing, and extension D. RNA polymerase to synthesize RNA primersarrow_forwardWhich of the following is true? a. Enhancers are proteins that bind to activator DNA sequences. b. Activators are proteins that bind to insulator sequences c. Repressors are proteins that bind to enhancer DNA sequences. d. Repressors are proteins which bind to silencer DNA sequencers.arrow_forwardWhich of the following plays a major part in determining whether or not RNA polymerase will transcribe a parti sequence? Select all that apply. A. A ribosome is already bonded to the gene sequence B. A complete set of transcription factors bind to DNA associated with that gene C. The chromatin structure is loosened in the region of that gene D. RNA polymerase binds to the promoter of that gene ..arrow_forward
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