How the blood flow from the right atrium to the right ventricle, and from right ventricle to lungs and back to the heart? Introduction: The heart is the muscular organ of the circulatory system of the vertebrates. The upper two chambers of the heart are known as the atria. Atrium has thin walls and collects the blood which returns to the heart. The lower chambers of the heart are known as the ventricles.

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Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…

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Chapter 15, Problem 5CA

Summary Introduction

To determine:

How the blood flow from the right atrium to the right ventricle, and from right ventricle to lungs and back to the heart?

Introduction:

The heart is the muscular organ of the circulatory system of the vertebrates. The upper two chambers of the heart are known as the atria. Atrium has thin walls and collects the blood which returns to the heart. The lower chambers of the heart are known as the ventricles.

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