Genetics: From Genes to Genomes, 5th edition
Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Chapter 15, Problem 33P
Summary Introduction

a.

To determine:

The number of genes and operons depicted in the figure, the average gene density, and the representativeness of the value to the most bacterial genomes.

Introduction:

The mentioned figure shows the RNA-Seq results from the RNA-Seq experiment. In the analysis part, the computer lines up each of cDNA sequence read with the genome sequence of the bacterium.

Summary Introduction

b.

To determine:

Whether the given data proves that t2110 is nonfunctional and the process by which scientists were able to assign a direction to the transcription when no transcript of the gene was found.

Introduction:

In the performed experiment, it was found that no transcripts for the gene t2110 were detected.

Summary Introduction

c.

To determine:

The evidence that suggests the existence of an attenuation or riboswitch mechanism, causing premature transcriptional termination of an operon and the observation of any of the operon that might be controlled by such a mechanism.

Introduction:

The process of attenuation is considered as the method of control in some bacterial operons, and it causes premature termination of transcription.

Summary Introduction

d.

To determine:

The depiction of the given figure if the antisense mechanism was involved in controlling the gene or operon.

Introduction:

The mentioned figure does not provide any evidence that any of the genes or operons are regulated by an antisense transcript.

Summary Introduction

e.

To determine:

The possibility of the transcription of the galM gene from the different promoter.

Introduction:

The given figure depicts galM gene to be a part of the operon containing galETK. There is even a possibility that galM is transcribed from a separate promoter.

Summary Introduction

f.

To determine:

Whether the data shown in the figure reveal the presence of a regulatory mechanism in relation to an sRNA occluding the ribosome binding site.

Introduction:

The ribosome binding site is present in upstream to the start codon. It is a sequence of nucleotides which play an important role in recruiting the ribosome at the initiation of the translation process.

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After you feel comfortable with your counting method and identifying cells in the various stages of mitosis, use the four images below of whitefish blastula to count the cells in each stage until you reach 100 total cells, recording your data below in Data Table 1. (You may not need to use all four images. Stop counting when you reach 100 total cells.) After totaling the cells in each stage, calculate the percent of cells in each stage. (Divide total of stage by overall total of 100 and then multiply by 100 to obtain percentage.)   Data Table 1Stage    Totals    PercentInterphase        Mitosis:        Prophase        Metaphase        Anaphase        Telophase        Cytokinesis        Totals    100    100% To find the length of time whitefish blastula cells spend in each stage, multiply the percent (recorded as a decimal, in other words take the percent number and divide by 100) by 24 hours. (Example: If percent is 20%, then Time in Hours = .2 * 24 = 4.8) Record your data in Data…

Chapter 15 Solutions

Genetics: From Genes to Genomes, 5th edition

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