Mindtap Biology, 1 Term (6 Months) Printed Access Card For Solomon/martin/martin/berg's Biology, 11th
11th Edition
ISBN: 9781337393096
Author: Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher: Cengage Learning
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Chapter 15, Problem 12TYU
Summary Introduction
To determine: The problems that might arise while producing eukaryotic protein in a bacterium and how the usage of transgenic plants or animals may help to solve these problems.
Concept introduction: Recombinant DNA technology allows addition of a eukaryotic gene into a bacterial plasmid vector. This recombinant plasmid expresses the eukaryotic gene by synthesizing a large quantity of protein encoded by it. There are certain hurdles in this, as the gene expression in eukaryotes and prokaryotes is different.
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I believe that there are many good things that can come out of people getting to design their baby’s genetic material. But there are also many bad things as well. From the article by Bio medical about the pros and cons of having a designer baby it states that a pro is that this type of engineering can “ might help prevent genetic diseases such as Alzheimer’s, Huntington’s Disease, down syndrome, Spinal Muscular Atrophy, and many others”. I think that it is great that we could get rid of Alzheimer’s due to how destructive it can be to the people that suffer it. But I think the other diseases that it can eliminate is horrible due to them making our world a more unique place such as people with autism, Down syndrome. By doing this it could eliminate the whole population of people with disabilities community and make everyone “normal”. Another bad that I found in the article Ethics of designer babies which states that a major flaw for these babies is “designer baby technologies suggest…
Chapter 15 Solutions
Mindtap Biology, 1 Term (6 Months) Printed Access Card For Solomon/martin/martin/berg's Biology, 11th
Ch. 15.1 - Prob. 1LOCh. 15.1 - Explain how gel electrophoresis is used to...Ch. 15.1 - Describe how PCR is used to amplify a specific...Ch. 15.1 - Compare the possible differences between a...Ch. 15.1 - Prob. 1CCh. 15.1 - Different forms of a protein are produced in the...Ch. 15.1 - What advantages does the PCR method have over gene...Ch. 15.2 - Describe the features of a typical CRISPR locus in...Ch. 15.2 - Explain the function of CRISPR in bacterial cells.Ch. 15.2 - Compare CRISPR-based endonucleases with...
Ch. 15.2 - Prob. 8LOCh. 15.2 - Prob. 1CCh. 15.2 - Prob. 2CCh. 15.2 - Prob. 3CCh. 15.3 - Prob. 9LOCh. 15.3 - Prob. 10LOCh. 15.3 - Discuss how qPCR, DNA microarrays (DNA chips), and...Ch. 15.3 - Explain how you would compare the expression of a...Ch. 15.3 - Prob. 2CCh. 15.4 - Describe how genome-wide association studies have...Ch. 15.4 - Explain how targeted gene silencing and knockout...Ch. 15.4 - Prob. 1CCh. 15.5 - Describe at least one important application of DNA...Ch. 15.5 - Prob. 1CCh. 15.5 - What are short tandem repeats (STRs), and why are...Ch. 15.5 - Why do gene targeting and mutagenesis screening in...Ch. 15.6 - Prob. 15LOCh. 15.6 - Prob. 16LOCh. 15.6 - Prob. 1CCh. 15.6 - Prob. 2CCh. 15.7 - Describe at least two safety issue associated with...Ch. 15.7 - What are some of the environment concerns...Ch. 15 - A plasmid (a) can be used as a DNA vector (b) is a...Ch. 15 - DNA molecules with complementary sticky ends...Ch. 15 - Prob. 3TYUCh. 15 - Which technique rapidly replicated specific DNA...Ch. 15 - Prob. 5TYUCh. 15 - A cDNA clone contains (a) introns (b) exons (c)...Ch. 15 - Prob. 7TYUCh. 15 - Gel electrophoresis separates nucleic acids on the...Ch. 15 - A CRISPR locus in a bacterium contains (a) short...Ch. 15 - DNA molecular with complementary sticky ends...Ch. 15 - These highly polymorphic molecular markers are...Ch. 15 - Prob. 12TYUCh. 15 - Prob. 13TYUCh. 15 - Prob. 14TYUCh. 15 - EVOLUTION LINK DNA technology, such as the...Ch. 15 - SCIENCE, TECHNOLOGY, AND SOCIETY What are some...
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- You just graduated from college and started working at a biotech startup called Scrofabulous. Your first job assignment is to clone the pig gene for the hormone prolactin. Assume that the pig gene for prolactin has not yet been isolated, sequenced, or mapped; What would be the most useful and economical first step to go about identifying and cloning the pig gene for prolactin? use the amino acid sequence of mouse prolactin to design a pair of degenerate oligonucleotide PCR primers to PCR-amplify the pig prolactin gene. RNAseq the pituitary gland of the pig, the most abundant gene is likely to to be prolactin Conduct a proteome search for peptides that match parts of mouse prolactin protein Sequence the pig genome, then translate the genome to find the gene predicted to encode for prolactin Crystalize the mouse prolactin protein and use Google's DeepMind Al to find the closest amino acid sequence in the pig proteomearrow_forwardWhat is the difference between a transgenic plant and a plant produced through selective breeding?arrow_forwardIt has been suggested that it would make the study of human diseases easier if cloned transgenic animals were produced that carried faulty versions of human genes (e.g., the gene that causes cystic fibrosis). a. Why would such animals be useful in medical research? : b. What ethical questions are raised by the creation of such transgenic animals?arrow_forward
- A PI in your facility has finished working with his transgenic line of mice and would like you to cryopreserve embryos (freeze them in liquid nitrogen) so that he can keep them for later experimentation without paying the extra cost of a breeding colony. Which of the following steps do you take? Select all that apply: a) Use the van der Lee/Boot and Whitten effect to time mate your females. b) Superovulate your females by injecting with PMSG and hCG. c) Breed the mice with a vasectomized male. d) Collect ova from the ampulla the day you find a plug. e) Collect blastocysts from the uterus 3 days after finding a plug.arrow_forwardDo all of them True/False 31) The process by which an electrical charge is used to introduce DNA into a cell to produce a transgenic organism is called electroporation.Answer: 32) Reproductive cloning is used to produce large amounts of mammalian proteins from transgenic agricultural animals such as cattle.Answer: 33) In gene addition, homologous recombination is used to remove the original gene and replace it with the cloned gene.Answer: 34) All stem cells have the potential to differentiateAnswer: 35) A bone marrow transplant involves the transfer of multipotent stem cellsAnswer: 36) The fact that in mammalian systems multiple genes may compensate for the loss of a gene is called gene redundancy.Answer:arrow_forwardLet’s say, you want to deliver a gene into a cell and in your lab, there are lot of options available to do that. Describe five different options that you can use to deliver that gene.arrow_forward
- What are the main differences between whole genome sequencing and whole exome sequencing?arrow_forwardBelow are some of the arguments about the use of transgenic organisms. From your own perspective, explain your answer in the questions in not more than 5 sentences. 1. If you are a farmer would you take the chance of growing crops that are pest resistant? Why or why not? 2. Considering the knowledge gained in genetic engineering, would you try to patronize GMO fruits and vegetables? Why or why not? 3. Is creating or altering genes of an organism a form of Blasphemy to the creator (God)? Why? 4. Is genetic engineering morally permissible or not?arrow_forwardWhat is a fluorophore? If you wanted to fluorescently label a brain cell, describe one way you could genetically label a brain cell using a transgenic approach and give one way you could genetically label a brain cell using a gene targeting homologous recombination approach.arrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license