PRINCIPLES OF LIFE W ACHIEVE
PRINCIPLES OF LIFE W ACHIEVE
3rd Edition
ISBN: 9781319505370
Author: HILLIS
Publisher: MAC HIGHER
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Chapter 15, Problem 1.2Q
Summary Introduction

To determine: The ways by which the statement can be supported that large genomes don not result in complex organisms.

Introduction: The genetic material in all the living organism is the DNA (deoxyribonucleic acid). All the eukaryotes as well the prokaryotes have defined set of DNA sequence, which is inherited from one generation to another and codes for all the characters of the organism.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
When setting up a PCR reaction to act as a negative control for the surface protein A gene... Which primers will you add to the reaction mix? mecA primers, spa primers, mecA primers and spa primers, no primers What will you add in place of template? sterile water, MRSA DNA, Patient DNA, S. aureus DNA
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Genome Annotation, Sequence Conventions and Reading Frames; Author: Loren Launen;https://www.youtube.com/watch?v=MWvYgGyqVys;License: Standard Youtube License