Prescott's Microbiology
Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 14.3, Problem 1MI

MICRO INQUIRY How does this attenuation respond to rates of protein synthesis in addition to the intracellular level of tryptophan?

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Measure the uptake of leucine by epithetial cells of the mouse intestine. Measurements of the rate of update of L-leucine, D-Leucine, and L-valine , with and without Na+ in the assay were perform and yield different results (see table below). A) What can you conclude about the properties and mechanism of leucine transporter? B) Would you expect L-leucine uptake to be inhibited by Ouabain, which is a cardiac glycoside drug treatment?
After purifying the expressed protien His6-NEP of interest, the different fractions collected will be run on an SDS-Page gels. If our purification was successful a band of correct size should be present in: A) orginal lysate B) lysate flowthrough C) wash flowthrough D) Elute E) orginal lystae and elite fractions
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Prescott's Microbiology

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