EBK BIOLOGY
EBK BIOLOGY
4th Edition
ISBN: 8220102797376
Author: BROOKER
Publisher: YUZU
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Chapter 14, Problem 8TY
Summary Introduction

Introduction: The causative agents which cause the phenomenon of mutation are called mutagens. Mutagens can cause the alteration in the genome of an organism, which, most of the times are fatal to the organism’s body. The presence of NER system in the organism’s body helps in the correction of defects produced in the strands of DNA.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
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