GENETICS:FROM GENES TO GENOMES(LL)-PKG
GENETICS:FROM GENES TO GENOMES(LL)-PKG
6th Edition
ISBN: 9781260377033
Author: HARTWELL
Publisher: MCG
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Chapter 14, Problem 3P

Now that the sequence of the entire E. coli K12 strain genome (roughly 5 Mb) is known, you can determine exactly where a cloned fragment of DNA came from in the genome by sequencing a few bases and matching that data with genomic information.

a. About how many nucleotides of sequence information would you need to determine exactly where a fragment is from?
b. If you had purified a protein from E. coli cells, roughly how many amino acids of that protein would you need to know to establish which gene encoded the protein?
c. You determine 100 nucleotides of sequence of genomic DNA from a different E. coli strain, but you cannot find a match in the E. coli K12 genome sequence. How is this possible?
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Alleles at the P locus control seed color. Plants which are pp have white seeds, white flowers and no pigment in vegetative parts. Plants which are P_ have black seeds, purple flowers and may have varying degrees of pigment on stems and leaves. Seed color can be assessed, visually, based on if the seed is white or not white A gene for mold resistance has been reported and we want to determine its inheritance and whether it is linked to P. For the purposes of this exercise, we will assume that resistance is controlled by a single locus M, and M_ plants are resistant and mm plants are susceptible.  Resistance can be measured, under greenhouse conditions, 2 weeks after planting, by injecting each seedling with a spore suspension. After two weeks, the seedlings can be rated as resistant or susceptible, based on whether or not tissue is actively sporulating. For this exercise we will use seed and data from the F10 generation of a recombinant inbred population produced using single seed…
Linkage in common bean Alleles at the P locus control seed color. Plants which are pp have white seeds, white flowers and no pigment in vegetative parts. Plants which are P_ have black seeds, purple flowers and may have varying degrees of pigment on stems and leaves. Seed color can be assessed, visually, based on if the seed is white or not white A gene for mold resistance has been reported and we want to determine its inheritance and whether it is linked to P. For the purposes of this exercise, we will assume that resistance is controlled by a single locus M, and M_ plants are resistant and mm plants are susceptible.  Resistance can be measured, under greenhouse conditions, 2 weeks after planting, by injecting each seedling with a spore suspension. After two weeks, the seedlings can be rated as resistant or susceptible, based on whether or not tissue is actively sporulating. For this exercise we will use seed and data from the F10 generation of a recombinant inbred population…
Alleles at the P locus control seed color. Plants which are pp have white seeds, white flowers and no pigment in vegetative parts. Plants which are P_ have black seeds, purple flowers and may have varying degrees of pigment on stems and leaves. Seed color can be assessed, visually, based on if the seed is white or not white A gene for mold resistance has been reported and we want to determine its inheritance and whether it is linked to P. For the purposes of this exercise, we will assume that resistance is controlled by a single locus M, and M_ plants are resistant and mm plants are susceptible.  Resistance can be measured, under greenhouse conditions, 2 weeks after planting, by injecting each seedling with a spore suspension. After two weeks, the seedlings can be rated as resistant or susceptible, based on whether or not tissue is actively sporulating. For this exercise we will use seed and data from the F10 generation of a recombinant inbred population produced using single seed…

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GENETICS:FROM GENES TO GENOMES(LL)-PKG

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