Biochemistry: Concepts and Connections
1st Edition
ISBN: 9780321839923
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 14, Problem 2P
When pure reduced cytochrome c is added to carefully prepared mitochondria along with ADP Pi, antimycin A, and oxygen, the cytochrome c becomes oxidized, and ATP is formed, with a P/O ratio approaching 1.0.
- Indicate the probable flow of electrons in this system.
- Why was antimycin A added?
- What does this experiment tell you about the location of coupling sites for oxidative phosphorylation?
- Write a balanced equation for the overall reaction (including cyt c oxidation and ATP synthesis).
- Calculate ΔGO' for the above reaction, using E'o values from Table 14.1 and a ΔGO' value for ATP hydrolysis of -32.2 kJ / mol.
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Chapter 14 Solutions
Biochemistry: Concepts and Connections
Ch. 14 - Prob. 1PCh. 14 - When pure reduced cytochrome c is added to...Ch. 14 - Freshly prepared mitochondria were incubated with...Ch. 14 - Prob. 4PCh. 14 - Prob. 5PCh. 14 - Intramitochondrial ATP concentrations are about 5...Ch. 14 - Prob. 7PCh. 14 - Prob. 8PCh. 14 - Years ago there was interest in using uncouplers...Ch. 14 - Prob. 10P
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