Genetics: From Genes to Genomes, 5th edition
Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Chapter 14, Problem 11P

The so-called hypervariable regions (HV1 and HV2) of the human mitochondrial genome are sometimes used in forensic analysis. They are two noncoding regions of the mitochondrial genome, each approximately 300 bp, that flank the origin of replication; the function of these DNA sequences is not well understood. However, these two regions of mtDNA show the most variation (SNPs and InDels) among different people. The DNA within HV1 and HV2 accumulates mutations at ten times the rate of DNA sequences in the nuclear genome.

a. Under what circumstances would human mtDNA be preferable over nuclear DNA for identifying individuals?
b. What are the disadvantages of using mtDNA, relative to nuclear DNA, in order to identify individuals?
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After you feel comfortable with your counting method and identifying cells in the various stages of mitosis, use the four images below of whitefish blastula to count the cells in each stage until you reach 100 total cells, recording your data below in Data Table 1. (You may not need to use all four images. Stop counting when you reach 100 total cells.) After totaling the cells in each stage, calculate the percent of cells in each stage. (Divide total of stage by overall total of 100 and then multiply by 100 to obtain percentage.)   Data Table 1Stage    Totals    PercentInterphase        Mitosis:        Prophase        Metaphase        Anaphase        Telophase        Cytokinesis        Totals    100    100% To find the length of time whitefish blastula cells spend in each stage, multiply the percent (recorded as a decimal, in other words take the percent number and divide by 100) by 24 hours. (Example: If percent is 20%, then Time in Hours = .2 * 24 = 4.8) Record your data in Data…
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Mitochondrial mutations; Author: Useful Genetics;https://www.youtube.com/watch?v=GvgXe-3RJeU;License: CC-BY