Genetics: A Conceptual Approach
Genetics: A Conceptual Approach
6th Edition
ISBN: 9781319050962
Author: Benjamin A. Pierce
Publisher: W. H. Freeman
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Chapter 13.2, Problem 17AQP

(a)

Summary Introduction

To determine:

The 5 and 3 end of the DNA template. As the given sequence of nucleotides bases is found in a DNA strand, which is single-stranded in nature:

ATTGCCAGATCATCCCAATAGAT

Introduction:

Transcription is the basic process of gene expression, in this process coping of information from DNA to RNA template. Both DNA and RNA are nucleic acids that form complementary bases with each other.

(b)

Summary Introduction

To determine:

The sequence and identify the 5 and 3 ends of the RNA transcribed from this template.

Introduction:

Transcription is the basic step of gene expression, in this process, a particular DNA segment is copied with the help of an enzyme called  RNA polymerase into RNA.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
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