EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Question
Chapter 13, Problem 9CT
Summary Introduction
To answer:
How many cycles it takes for the T4 bacteriophages to infect the colony of 1.5 billion E. coli.
Introduction:
The viruses that invade the bacterial cell are termed as bacteriophages. The most common known bacteriophages are T4 bacteriophages. These bacteriophages have a complex icosahedral head that contains the genetic material and tail fiber like a hypodermic needle. Through the tail, they infect the bacteria with their genome.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
If a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis, yields 185 viable viruses, how many phages will exist in a single plaque if 5 more lytic cycles occur?
Express your answer using three significant figures (example: 1.11×10^3).
(there are multiple right answers to this homework question but I can't tell which one is right)
You are designing a phage therapy for a cystic fibrosis patient with an multi-antibiotic resistant Mycobacterium infection. The Mycobacterium infection is preventing the patient from taking immunosuppressant drugs that are needed for a successful lung transplant. The idea is that introducing the right phage to the patient will kill the Mycobacterium cells and allow the patient to go on immunosuppressant drugs that will ensure the new lung is not rejected by the body.
In the first step of your phage therapy design process, you isolate the exact Mycobacterium strain from the patient. What is the most important thing must you establish next about this bacterial isolate?
That the isolate is genomically stable so you can study it
That the isolate is similar to Mycobacterium in other patients
That the isolate rapidly evolves due to horizontal gene transfer
That the Mycobacterium is…
Consider a hypothetical phage whose DNA replicates exclusively by rolling circle replication. A phage with radioactive DNA in both strands infects a bacterium and is allowed to replicate in a nonradioactive medium. Assume that only daughter DNA from the elongated branch ever gets packaged into progeny particles.
What fraction of the parental radioactivity will appear in the progeny phage?
How many progeny phage will contain radioactive DNA?
What is the fundamental difference between the initiation of θ replication and that of the rolling circle?
Chapter 13 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 13 - Why are naked icosahedral viruses able to...Ch. 13 - What characteristics of the genomes of...Ch. 13 - Prob. 3TMWCh. 13 - Prob. 1EDCSCh. 13 - Prob. 4TMWCh. 13 - Prob. 5TMWCh. 13 - Prob. 6TMWCh. 13 - Prob. 7TMWCh. 13 - Prob. 1CCSCh. 13 - Prob. 1MC
Ch. 13 - Prob. 2MCCh. 13 - Prob. 3MCCh. 13 - Prob. 4MCCh. 13 - Prob. 5MCCh. 13 - Prob. 6MCCh. 13 - Prob. 7MCCh. 13 - Prob. 8MCCh. 13 - Prob. 9MCCh. 13 - Prob. 10MCCh. 13 - Prob. 1MCh. 13 - Prob. 1VICh. 13 - Prob. 2VICh. 13 - Prob. 1SACh. 13 - Prob. 2SACh. 13 - Prob. 3SACh. 13 - Prob. 4SACh. 13 - Prob. 5SACh. 13 - What is the difference between a virion and a...Ch. 13 - How is a provirus like a prophage? How is it...Ch. 13 - Prob. 8SACh. 13 - Prob. 9SACh. 13 - Prob. 10SACh. 13 - Prob. 1CTCh. 13 - Prob. 2CTCh. 13 - Prob. 3CTCh. 13 - Prob. 4CTCh. 13 - Prob. 5CTCh. 13 - Prob. 6CTCh. 13 - Prob. 7CTCh. 13 - Why has it been difficult to develop a complete...Ch. 13 - Prob. 9CTCh. 13 - What differences would you expect in the...Ch. 13 - Prob. 11CTCh. 13 - Prob. 12CTCh. 13 - Prob. 13CTCh. 13 - Using the following terms, fill in the following...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- 10 mL of a bacteriophage suspension is mixed with 5 mL of a bacterial culture and 25% of the phages adsorb. The bacteriophage suspension has a concentration of 4x1018 viruses per mL, and the bacterial culture had a concentration of 6x1014 bacteria per mL. How many viruses are adsorbed per cell (multiplicity of infection)? What fraction of the cells is uninfected? What fraction of the cells is single infected? What fraction of the cells is multiple infected?arrow_forwardIf a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis, yields 200 viable viruses, how many phages will exist in a single plaque if three more lytic cycles occur?arrow_forwardIn Hershey-Chase experiment, bacteriophages protein coats were tagged with radioactive isotope S-32. These phages were used to infect E. coli cells and the cells were further centrifuged to form pellets. Why was the radioactivity level of S-32 found greater outside the cells compared to the E. coli cell pellets? Explain briefly. If the experiment is repeated in the same manner but this time the phage protein coats are labelled with isotope X and the phage DNA with isotope Y, which isotope’s radioactivity will be found in greater amounts in the E. coli cell pellets after centrifugation? Explain briefly.arrow_forward
- Most temperate phages integrate into the host chromosome, whereas some replicate as plasmids. Which kind of relationship would you think would be more likely to maintain the phage in the host cell? Why?arrow_forwardHost DNA is digested into small pieces, which are occasionally assembled with phage proteins, creating a phage with bacterial chromosomal DNA. If the breakage of the chromosomal DNA is not random (i.e., it is more likely tobreak at certain spots as opposed to other spots), how might suchnonrandom breakage affect cotransduction frequency?arrow_forwardAssume you isolate a single stranded (+) RNA virus. When you examine the proteins in the virus, you find that it does NOT contain replicase enzymes within its capsid. Which of the following is true? This virus must have a gene that encodes replicase. This virus will not be able to enter a host cell. Its genome cannot be translated (the process of translation) by the host cell ribosomes. A DNA copy of the viral genome has to be made before viral genes are expressed. This virus must lack surface antigens.arrow_forward
- Which of the statements is TRUE about conjugation? a) Plasmid DNA is transferred from a donor (F+) cell b) It may only be performed by competent cells c) DNA is always integrated into recipient chromosome d) It may be specialized or generalizedarrow_forwardOne mL of a bacteriophage suspension is mixed with 20 mL of a bacterialculture and 50% of the phages adsorb. We know that the bacteriophagesuspension had a concentration of 1x10^10 viruses per mL, and the bacterialculture had a concentration of 3x10^8 bacteria per mL. How many viruses are adsorbed per cell (multiplicity of infection)?arrow_forwardWhich of the following statements is not applicable to conjugation? A) OF factors are transferrable plasmids passed from one cell to another during conjugation. B) O During conjugation, rolling circle replication transfers an F factor from an F+ cell to an F- cell. ) O An F factor acquired during conjugation will automatically be integrated into the recipient's chromosome. D) O Conjugation requires physical contact between cells via the formation of a sex pilus. E) O An Hfr strain is capable of transferring a portion of the chromosome during conjugation.arrow_forward
- When various strains of λ phage are seeded on a lawn of E. coli, they can form clear or turbid plaques. (b) For mutant λ phages that can only form clear plaques, give two different types of mutation in the phage that can explain the clear plaque phenotype.arrow_forwardIn Figure 5-2, in which of the four processes shown can acomplete bacterial genome be transferred from one cellto another?arrow_forwardYou are designing a phage therapy for a cystic fibrosis patient with an multi-antibiotic resistant Mycobacterium infection. The Mycobacterium infection is preventing the patient from taking immunosuppressant drugs that are needed for a successful lung transplant. The idea is that introducing the right phage to the patient will kill the Mycobacterium cells and allow the patient to go on immunosuppressant drugs that will ensure the new lung is not rejected by the body. You need to find a phage that infects the Mycobacterium you isolated from the patient. Which TWO options have the highest chance of success? A. Screening a colleague's library of known Mycobacterium phages for phage that infect the patient's Mycobacterium isolate b. Choosing likely candidates from analysis of phage 16S rRNA phylogeny in the tree of life C. Isolating new phage from soil using a plaque assay with the Mycobacterium isolate D. Obtaining a very well-studied phage, such as T4 bacteriophage, that…arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
What Is A Virus ? ; Author: Peekaboo Kidz;https://www.youtube.com/watch?v=YS7vsBgWszI;License: Standard YouTube License, CC-BY