Microbiology with Diseases by Body System (5th Edition)
5th Edition
ISBN: 9780134477206
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 13, Problem 9CT
Summary Introduction
To answer:
How many cycles it takes for the T4 bacteriophages to infect the colony of 1.5 billion E. coli.
Introduction:
The viruses that invade the bacterial cell are termed as bacteriophages. The most common known bacteriophages are T4 bacteriophages. These bacteriophages have a complex icosahedral head that contains the genetic material and tail fiber like a hypodermic needle. Through the tail, they infect the bacteria with their genome.
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If a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis, yields 185 viable viruses, how many phages will exist in a single plaque if 5 more lytic cycles occur?
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Chapter 13 Solutions
Microbiology with Diseases by Body System (5th Edition)
Ch. 13 - Why are naked icosahedral viruses able to...Ch. 13 - What characteristics of the genomes of...Ch. 13 - Prob. 3TMWCh. 13 - Prob. 1EDCSCh. 13 - Prob. 4TMWCh. 13 - Prob. 5TMWCh. 13 - Prob. 6TMWCh. 13 - Prob. 7TMWCh. 13 - Prob. 1CCSCh. 13 - Prob. 1MC
Ch. 13 - Prob. 2MCCh. 13 - Prob. 3MCCh. 13 - Prob. 4MCCh. 13 - Prob. 5MCCh. 13 - Prob. 6MCCh. 13 - Prob. 7MCCh. 13 - Prob. 8MCCh. 13 - Prob. 9MCCh. 13 - Prob. 10MCCh. 13 - Prob. 1MCh. 13 - Prob. 1VICh. 13 - Prob. 2VICh. 13 - Prob. 1SACh. 13 - Prob. 2SACh. 13 - Prob. 3SACh. 13 - Prob. 4SACh. 13 - Prob. 5SACh. 13 - What is the difference between a virion and a...Ch. 13 - How is a provirus like a prophage? How is it...Ch. 13 - Prob. 8SACh. 13 - Prob. 9SACh. 13 - Prob. 10SACh. 13 - Prob. 1CTCh. 13 - Prob. 2CTCh. 13 - Prob. 3CTCh. 13 - Prob. 4CTCh. 13 - Prob. 5CTCh. 13 - Prob. 6CTCh. 13 - Prob. 7CTCh. 13 - Why has it been difficult to develop a complete...Ch. 13 - Prob. 9CTCh. 13 - What differences would you expect in the...Ch. 13 - Prob. 11CTCh. 13 - Prob. 12CTCh. 13 - Prob. 13CTCh. 13 - Using the following terms, fill in the following...
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- Consider a hypothetical phage whose DNA replicates exclusively by rolling circle replication. A phage with radioactive DNA in both strands infects a bacterium and is allowed to replicate in a nonradioactive medium. Assume that only daughter DNA from the elongated branch ever gets packaged into progeny particles. What fraction of the parental radioactivity will appear in the progeny phage? How many progeny phage will contain radioactive DNA? What is the fundamental difference between the initiation of θ replication and that of the rolling circle?arrow_forwardDuring the lysogenic cycle of lambda (1) phage the phage genome is integrated in the host chromosome where it is passed on to daughter cells through binary fission. the phage DNA is copied and accumulates in the cytoplasm. Then a trigger causes capsid proteins to be made and phages are assembled. the product of one prophage gene is made and it activates most of the other prophage genes. the viral genes immediately turn the host cell into a lambda-producing factory. Then the host lyses. 0000arrow_forward10 mL of a bacteriophage suspension is mixed with 5 mL of a bacterial culture and 25% of the phages adsorb. The bacteriophage suspension has a concentration of 4x1018 viruses per mL, and the bacterial culture had a concentration of 6x1014 bacteria per mL. How many viruses are adsorbed per cell (multiplicity of infection)? What fraction of the cells is uninfected? What fraction of the cells is single infected? What fraction of the cells is multiple infected?arrow_forward
- If a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis, yields 200 viable viruses, how many phages will exist in a single plaque if three more lytic cycles occur?arrow_forwardIn Hershey-Chase experiment, bacteriophages protein coats were tagged with radioactive isotope S-32. These phages were used to infect E. coli cells and the cells were further centrifuged to form pellets. Why was the radioactivity level of S-32 found greater outside the cells compared to the E. coli cell pellets? Explain briefly. If the experiment is repeated in the same manner but this time the phage protein coats are labelled with isotope X and the phage DNA with isotope Y, which isotope’s radioactivity will be found in greater amounts in the E. coli cell pellets after centrifugation? Explain briefly.arrow_forwardMost temperate phages integrate into the host chromosome, whereas some replicate as plasmids. Which kind of relationship would you think would be more likely to maintain the phage in the host cell? Why?arrow_forward
- The figure depicts five stages of the lytic cycle. ********* Select the statement that describes stage 3 of the lytic cycle, as shown in the diagram. The host cell synthesizes cellular proteins to prepare for lysis. The host cell incorporates phage DNA into its own genome. The host cell replicates the phage DNA. O The host cell synthesizes proteins that degrade phage DNA.arrow_forwardHost DNA is digested into small pieces, which are occasionally assembled with phage proteins, creating a phage with bacterial chromosomal DNA. If the breakage of the chromosomal DNA is not random (i.e., it is more likely tobreak at certain spots as opposed to other spots), how might suchnonrandom breakage affect cotransduction frequency?arrow_forwardAssume you isolate a single stranded (+) RNA virus. When you examine the proteins in the virus, you find that it does NOT contain replicase enzymes within its capsid. Which of the following is true? This virus must have a gene that encodes replicase. This virus will not be able to enter a host cell. Its genome cannot be translated (the process of translation) by the host cell ribosomes. A DNA copy of the viral genome has to be made before viral genes are expressed. This virus must lack surface antigens.arrow_forward
- Which of the statements is TRUE about conjugation? a) Plasmid DNA is transferred from a donor (F+) cell b) It may only be performed by competent cells c) DNA is always integrated into recipient chromosome d) It may be specialized or generalizedarrow_forwardWhich of the following statements describes the lysogenic cycle of lambda (A) phage? After infection, the viral genes immediately turn the host cell into a lambda-producing factory, and the host cell then lyses. Most of the prophage genes are activated by the product of a particular prophage gene. The phage DNA is incorporated into the bacteria DNA The phage DNA is copied and exits the cell as a phage. The phage DNA get destructed by the host bacteria DNAarrow_forwardOne mL of a bacteriophage suspension is mixed with 20 mL of a bacterialculture and 50% of the phages adsorb. We know that the bacteriophagesuspension had a concentration of 1x10^10 viruses per mL, and the bacterialculture had a concentration of 3x10^8 bacteria per mL. How many viruses are adsorbed per cell (multiplicity of infection)?arrow_forward
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