Chapter 13, Problem 34A

Summary Introduction

To examine:

The banding patterns and match each individual with the mosquito that bit him or her.

Introduction:

The technique of separating DNA fragments based on their size is termed as gel electrophoresis. In this technique, DNA samples are added to well at the end of the gel. Then, electric current is applied through the gel. As DNA is negatively charged, DNA fragments go towards the positive electrode.

Explanation of Solution

From A, B, and C individuals DNA fingerprints of mosquitoes were obtained. These DNA fingerprints were matched with the types of mosquitoes ranging from 1-8 using a gel electrophoresis. After examining the given gel, it was seen, that first DNA fingerprint obtained from A individual matched with DNA obtained from 2, 3, 4, 6, and 7. As, the gel was examined further, it was observed that banding patterns of 3 and 6 number mosquitoes were matching aith A individual DNA fingerprint. So, A was bitten by 3 and 6 number mosquitoes.

The banding pattern of B is matching only with 5 number mosquitos, and the banding pattern of C is matching only with 7 number mosquito.