Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Chapter 1.3, Problem 2MQ
Summary Introduction
The unicellular organisms are considered as the oldest form of lives that are first evolved on the earth about 4.6 billion years ago. In biological classification, the three domain system of life is introduced by Carl Woese and his colleagues in 1977.
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19. On the diagram below
a. Label the three pictures as: DNA; polypeptide; or RNA.
b. Label the arrows as: translation or transcription/RNA processing.
c. Add the following details to the diagram.
Promoter region
TATA box
Transcription start site
Transcription terminator
Intron (A,B,C,D)
Exons (1,2,3,4,5)
Splice sites
5' cap
5' UTR (untranslated region)
3' poly A tail
3' UTR (untranslated region)
Translational start (AUG)
Translational stop (UGA, UAG, or UAA)
N and C ends of polypeptide
0000
Match the letter labels in the figure below to the terms. Some letter
labels are not used.
MNNNNNNIN
M
C
B
A
M
D
F
E
H
K
G
8
The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
Chapter 1 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 1.1 - In what ways are microorganisms important to...Ch. 1.1 - Why are microbial cells useful for understanding...Ch. 1.1 - What is a microbial colony and how is one formed?Ch. 1.1 - What are bacterial colonies and how are they...Ch. 1.2 - What structures are universal to all types of...Ch. 1.2 - Prob. 2MQCh. 1.2 - What structures can be used to distinguish between...Ch. 1.2 - Prob. 1CRCh. 1.3 - How old is Earth and when did cells first appear...Ch. 1.3 - Prob. 2MQ
Ch. 1.3 - Why were cyanobacteria so important in the...Ch. 1.3 - Prob. 1CRCh. 1.4 - Prob. 1MQCh. 1.4 - Prob. 2MQCh. 1.4 - Prob. 3MQCh. 1.4 - Prob. 1CRCh. 1.5 - Define the terms magnification and resolution.Ch. 1.5 - Prob. 2MQCh. 1.5 - Prob. 1CRCh. 1.6 - Prob. 1MQCh. 1.6 - Prob. 2MQCh. 1.6 - How can cells be made to fluoresce?Ch. 1.6 - Prob. 1CRCh. 1.7 - Prob. 1MQCh. 1.7 - Prob. 2MQCh. 1.7 - Prob. 1CRCh. 1.8 - Prob. 1MQCh. 1.8 - Prob. 2MQCh. 1.8 - Prob. 1CRCh. 1.9 - Prob. 1MQCh. 1.9 - Prob. 2MQCh. 1.9 - Besides ending the controversy over spontaneous...Ch. 1.9 - Explain the principle behind the Pasteur flask in...Ch. 1.10 - How do Kochs postulates ensure that cause and...Ch. 1.10 - What advantages do solid media offer for the...Ch. 1.10 - Prob. 3MQCh. 1.10 - Prob. 1CRCh. 1.11 - What is meant by the term enrichment culture?Ch. 1.11 - Prob. 2MQCh. 1.11 - What were the major microbiological interests of...Ch. 1.12 - Describe the experiments that proved DNA was the...Ch. 1.12 - Why are microbial cells useful tools for basic...Ch. 1.12 - Describe the experiments that proved DNA to be the...Ch. 1.13 - What kinds of evidence support the three-domain...Ch. 1.13 - What is a phylogenetic tree?Ch. 1.13 - List three reasons why rRNA genes are suitable for...Ch. 1.13 - What insights led to the reconstruction of the...Ch. 1.14 - How are viruses different from Bacteria, Archaea,...Ch. 1.14 - What four bacterial phyla contain the most...Ch. 1.14 - Prob. 3MQCh. 1.14 - What features (or lack of features) can be used to...Ch. 1 - Pasteurs experiments on spontaneous generation...Ch. 1 - Describe the lines of proof Robert Koch used to...Ch. 1 - Imagine that all microorganisms suddenly...
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