
Biochemistry (Looseleaf)
9th Edition
ISBN: 9781319114800
Author: BERG
Publisher: MAC HIGHER
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Question
Chapter 13, Problem 26P
Interpretation Introduction
Interpretation:
The reason for the efficiency of the enzyme to be physiologically crucial needs to be explained.
Concept introduction:
Acetylcholine act as a neurotransmitter which means it sends messages or chemicals from one cell to another cell of the body. Acetylcholinesterase helps in stopping the excitation of a nerve cell after the transmission of impulse or in other words, this enzyme causes rapid hydrolysis of acetylcholine.
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Classify each phrase as describing kinases, phosphatases, neither, or both.
Kinases
Phosphatases
Neither
Both
Answer Bank
transfer phosphoryl groups to acidic amino acids in eukaryotes
may use ATP as a phosphoryl group donor
remove phosphoryl groups from proteins
catalyze reactions that are the reverse of dephosphorylation reactions
regulate the activity of other proteins
catalyze phosphorylation reactions
PKA as an example
turn off signaling pathways triggered by kinases
Consider the reaction.
kp
S P
kg
What effects are produced by an enzyme on the general reaction?
AG for the reaction increases.
The rate constant for the reverse reaction (kr) increases.
The reaction equilibrium is shifted toward the products.
The concentration of the reactants is increased.
The activation energy for the reaction is lowered.
The formation of the transition state is promoted.
The graph displays the activities of wild-type and several mutated forms of subtilisin on a logarithmic scale.
The mutations are identified as:
• The first letter is the one-letter
abbreviation for the amino acid
being altered.
• The number identifies the
position of the residue in the
primary structure.
⚫ The second letter is the
one-letter abbreviation for the
amino acid replacing the
original one.
• Uncat. refers to the estimated
rate for the
uncatalyzed reaction.
Log₁(S-1)
Wild type
S221A
H64A
-5
D32A
S221A
H64A
D32A
-10
Uncat.
How would the activity of a reaction catalyzed by a version of subtilisin with all three residues in the catalytic triad
mutated compare to the activity of the uncatalyzed reaction?
It would have more activity, because the reaction catalyzed by the triple mutant is approximately three-fold faster
than the uncatalyzed reaction.
It would have less activity, because the reaction catalyzed by the triple mutant is approximately 1000-fold slower
than the…
Chapter 13 Solutions
Biochemistry (Looseleaf)
Ch. 13 - Prob. 1PCh. 13 - Prob. 2PCh. 13 - Prob. 3PCh. 13 - Prob. 4PCh. 13 - Prob. 5PCh. 13 - Prob. 6PCh. 13 - Prob. 7PCh. 13 - Prob. 8PCh. 13 - Prob. 9PCh. 13 - Prob. 10P
Ch. 13 - Prob. 11PCh. 13 - Prob. 12PCh. 13 - Prob. 13PCh. 13 - Prob. 14PCh. 13 - Prob. 15PCh. 13 - Prob. 16PCh. 13 - Prob. 17PCh. 13 - Prob. 18PCh. 13 - Prob. 19PCh. 13 - Prob. 20PCh. 13 - Prob. 21PCh. 13 - Prob. 22PCh. 13 - Prob. 23PCh. 13 - Prob. 24PCh. 13 - Prob. 25PCh. 13 - Prob. 26PCh. 13 - Prob. 27PCh. 13 - Prob. 28PCh. 13 - Prob. 29PCh. 13 - Prob. 30PCh. 13 - Prob. 31PCh. 13 - Prob. 32P
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