Concept explainers
Using the components in the accompanying diagram, design regulatory modules (i.e., enhancer
a. How will the gene be activated in the proper cell type?
b. How will its expression be maintained?
c. How will expression be prevented in other cell types?
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Genetic Analysis: An Integrated Approach (3rd Edition)
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- Tumor-suppressor genes are normal human genes that prevent uncontrollable cell growth. Starting with a normal laboratory humancell line, describe how you could use transposon tagging to identifytumor-suppressor genes. (Note: When a TE hops into a tumorsuppressorgene, it may cause uncontrolled cell growth. This is detected as a large clump of cells among a normal monolayer of cells.)arrow_forwardDuring certain stages of cell growth, the requirement for certain gene products may require gene amplification. What purpose does gene amplification serve?arrow_forwardPlease explainarrow_forward
- Members of a family are affected by a hereditary inflammatory and immunodeficiency disease. The disease phenotype was due to a mutation in the PLCG2 gene, determined by comparing an affected father and an affected daughter, both heterozygotes, with the homozygous unaffected mother (Zhou et al, 2012). The wild-type protein acts to regulate various immune and inflammatory pathways within the cell. The variant protein is over expressed (more abundant), leading to increased intracellular signaling and leading to increased inflammation and reduced immunity in the affected people. Select all the statements below that can be correctly applied to this system. [Hint: There are FOUR.] O co-dominance dominant negative mutant allele O gain-of-function mutation O haplosufficent wildtype allele O haploinsuffient wildtype allele O leaky mutant allele O loss-of-function mutation O null mutant allele O partial dominance O simple dominance O the mutant allele is hypermorphic O the mutant allele is…arrow_forwardPlease explainarrow_forwardFlowering Locus C (FLC) is a gene that is responsible for flowering in certain plants. FLC is normally expressed in new seedlings, which prevents flowering. When a plant is exposed to cold temperatures, FLC expression decreases and the plant produces flowers. FLC is regulated through epigenetic modifications. Based on this information, you should expect which of the following in histone proteins near the FLC gene: 1.In new seedlings, histones will be acetylated; while in the cold, histone methylation occurs 2.In new seedlings, histones will be methylated; while in the cold, histone acetylation occurs 3.In new seedlings, histones will be methylated; while in the cold, histone deacetylation occursarrow_forward
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