Genetic Analysis: An Integrated Approach (3rd Edition)
Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
bartleby

Videos

Question
Book Icon
Chapter 13, Problem 18P
Summary Introduction

To analyze:

The expression of UG 4 gene occurs in leaf and stem tissue of plant Arabidopsis thaliana. To study the process of regulation of UG 4 gene expression, six small deletions are introduced at the DNA sequence upstream of the gene coding sequence. The site of deletions and their impacts on UG 4 gene expression are shown in the figure of question.

From the given information, following questions are asked.

Explanation on reason for different impacts of deletions B and F on expression in stem tissue and leaf tissue.

Explanation on reason for deletion of D allows UG 4 gene expression in leaf tissue but not in stem tissue.

Explanation on reason for deletion of E reduces UG 4 gene expression in leaf tissue but not in stem tissue.

Introduction:

A eukaryotic gene has regulatory sequences which comprises of promoters, enhancers, and silencers which collectively control the gene expression.

Enhancers are present upstream of the promoter sequence. Binding of the enzymes and the transcription factor to the enhancer sequences bring about the regulation of the transcription of the gene. Enhancers are termed as positive cis acting elements as they act on the gene present on the same strand; it can also affect the gene which is 1000 bp away. Enhancers aid in increasing the basal level transcription of the gene expression while the silencers are the negative regulatory sequences which bind to the repressor sequences keeping the gene in the repressed state.

Promoter is the sequence of nucleotides on DNA where RNA polymerase is recruited to carry out the transcription of the particular gene. Promoters’ sequences are observed to highly conserve through evolution therefore calling them consensus sequences. In prokaryotes, the promoter comprises of the sequence called as TATA BOX (TATAAT) at -10 and PRIBNOW BOX at -35(TTGACA). Promoter and Enhancer present in the upstream together bring out the maximum expression of the gene.

Blurred answer
Students have asked these similar questions
WRITTEN WORK 3: NON-MENDELIAN GENETICS Part A: Complete the Punnett square and calculate for the probability of genotype and phenotype. i i Genotype: Phenotype: 08:55
1:42 PM ១ 99% Apart from food, plants need other nutrients like water and minerals. Nitrogen, a mineral, is an important part of all living cells. All organisms need nitrogen in order to grow and repair. Although nitrogen exists in its elemental form in the atmosphere, it cannot be directly used by plants. 7 Where else can plants obtain their nitrogen from? Plants make their own nitrogen. B Plants get it from animals. Plants get it from the soil. D Plants have special structures to break down atmospheric nitrogen. v3.7.63.140.4 | 6763e9417a3dbb80fa0f87b2 | Dec 19, 2024 | 3:07 PM | 84126 | en_8
Compare the cloning efficiencies: SmaI vs. EcoRI.
Knowledge Booster
Background pattern image
Biology
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
SEE MORE QUESTIONS
Recommended textbooks for you
Text book image
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Text book image
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Text book image
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Text book image
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Text book image
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Text book image
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
What are Mutations and what are the different types of Mutations?; Author: Science ABC;https://www.youtube.com/watch?v=I16YlE8qTBU;License: Standard youtube license