Campbell Essential Biology with Physiology (6th Edition)
Campbell Essential Biology with Physiology (6th Edition)
6th Edition
ISBN: 9780134711751
Author: Eric J. Simon, Jean L. Dickey, Jane B. Reece
Publisher: PEARSON
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Chapter 12, Problem 9SQ
Summary Introduction

To determine: The correct order of steps of human gene therapy.

Introduction: Human gene therapy is used for the treatment of diseases but inserting new or altered gene in affected person. In some cases where the disease is caused by the presence of mutant allele, the therapy is done by replacing the mutant allele with normal allele.

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You have identified a new species of a Gram-positive bacteria. You would like to screen their genome for all proteins that are covalently linked to the cell wall.   You have annotated the genome, so that you identified all the promoters, operons, and genes sequences within the operons.   Using these features, what would you screen for to identify a set of candidates for proteins covalently linked to the bacterial cell wall.
Below is a diagram from a genomic locus of a bacterial genome. Each arrow represents a coding region, and the arrowheads indicate its orientation in the genome. The numbers are randomly assigned. Draw the following features on the diagram, and explain your rationale for each feature: 10 12 合會會會會長 6 a) Expected transcriptions, based on known properties of bacterial genes and operons. How many proteins are encoded in each of the transcripts? b) Location of promoters (include rationale) c) Location of transcriptional terminators (include rationale) d) Locations of Shine-Dalgarno sequences (include rationale)
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