Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 12, Problem 4P
Interpretation Introduction

Interpretation:

The diffusion coefficient at Biochemistry, Chapter 12, Problem 4P , additional homework tip  1if protein in a membrane of 100 KDa has an effective viscosity of 1 poise and the average distance traveled by 100 kDa protein in Biochemistry, Chapter 12, Problem 4P , additional homework tip  2Biochemistry, Chapter 12, Problem 4P , additional homework tip  3and Biochemistry, Chapter 12, Problem 4P , additional homework tip  4needs to be calculated.

Concept introduction:

Diffusion coefficient also called diffusivity is the diffusing quantity from one region to another passing through unit cross-section area per unit time. It is denoted by D.

Biochemistry, Chapter 12, Problem 4P , additional homework tip  5

Where,

K =Stefan Boltzmann constant Biochemistry, Chapter 12, Problem 4P , additional homework tip  6

T = Absolute temperature

Biochemistry, Chapter 12, Problem 4P , additional homework tip  7viscosity of the solvent

r = radius of the sphere.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)
a) What are the differences between the Direct & Indirect Immunofluorescence Assays?  b) What are the advantages of the Indirect Immunofluorescence Assays?  c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?
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