GENETICS:FROM GENES TO GENOMES(LL)-PKG
6th Edition
ISBN: 9781260377033
Author: HARTWELL
Publisher: MCG
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Chapter 12, Problem 35P
A number of yeast-derived elements were added to the circular bacterial plasmid pBR322. Yeast that require uracil for growth (Ura- cells) were transformed with these modified plasmids and Ura+ colonies were selected by growth in media lacking uracil. For plasmids containing each of the elements listed in parts (a) to (c), indicate whether you expect the plasmid to integrate into a chromosome by recombination, or instead whether it is maintained separately as a plasmid. If the plasmid is maintained autonomously, is it stably inherited by all of the daughter cells of subsequent generations when you no longer select for Ura+ cells (that is, when you grow the yeast in media containing uracil)?
| a. | URA+ gene |
| b. | URA+ gene, ARS |
| c. | URA+ gene, ARS, CEN (centromere) |
| d. | What would need to be added in order for these sequences to be maintained stably in yeast cells as a linear artificial chromosome? |
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Chapter 12 Solutions
GENETICS:FROM GENES TO GENOMES(LL)-PKG
Ch. 12 - For each of the terms in the left column, choose...Ch. 12 - Many proteins other than histones are found...Ch. 12 - What difference exists between the compaction of...Ch. 12 - What is the role of the core histones in...Ch. 12 - a. About how many molecules of histone H2A would...Ch. 12 - The enzyme micrococcal nuclease can cleave...Ch. 12 - a. What letters are used to represent the short...Ch. 12 - About 2000 G bands are visible in a...Ch. 12 - Suppose you performed a fluorescence in situ...Ch. 12 - Which of the following would be suggested by a...
Ch. 12 - For each of the following pairs of chromatin...Ch. 12 - a. Drosophila b. Humans Give examples of...Ch. 12 - One histone modification that is seen consistently...Ch. 12 - Recently, scientists constructed a transgene that...Ch. 12 - Drosophila geneticists have isolated many...Ch. 12 - On the following figures, genes A and B are on the...Ch. 12 - Prob. 17PCh. 12 - The first page of this chapter displays photos of...Ch. 12 - The human genome contains about 3 billion base...Ch. 12 - The mitotic cell divisions in the early embryo of...Ch. 12 - In an experiment published in the journal Cell in...Ch. 12 - a. What DNA sequences are found at the telomeres...Ch. 12 - Prob. 23PCh. 12 - a. In a fluorescent in situ hybridization FISH...Ch. 12 - If you are comparing the two telomeres in each...Ch. 12 - a. What DNA sequences are commonly found at human...Ch. 12 - On the graphs presented in Problem 21, no data is...Ch. 12 - Prob. 29PCh. 12 - Prob. 30PCh. 12 - In the 1920s, Barbara McClintock, later a Nobel...Ch. 12 - Give at least one example of a chromosomal...Ch. 12 - Cornelia de Lange syndrome CdLS is a rare human...Ch. 12 - a. Give at least three examples of types of...Ch. 12 - A number of yeast-derived elements were added to...Ch. 12 - Prob. 36PCh. 12 - The completely synthetic yeast chromosome Syn III...
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