Pearson eText Genetic Analysis: An Integrated Approach -- Instant Access (Pearson+)
3rd Edition
ISBN: 9780135564172
Author: Mark Sanders, John Bowman
Publisher: PEARSON+
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Chapter 12, Problem 28P
How would mutations that inactivate each of the following genes affect the determination of the lytic or lysogenic life cycle in mutated
a. cIc.croe. cII and cro
b. cIId.intf. N
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The figure above shows a schematic of genes and transcription control elements from phage λ. Use this figure as an aid to help you describe the molecular events involved in:
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Chapter 12 Solutions
Pearson eText Genetic Analysis: An Integrated Approach -- Instant Access (Pearson+)
Ch. 12 - 12.1 Bacterial genomes frequently contain groups...Ch. 12 - Transcriptional regulation of operon gene...Ch. 12 - Why is it essential that bacterial cells be able...Ch. 12 - Identify similarities and differences between an...Ch. 12 - The transcription of -galactosidase and permease...Ch. 12 - 12.6 Is attenuation the product of an allosteric...Ch. 12 - The trpL region contains four repeated DNA...Ch. 12 - The CAP binding site in the lac promoter is the...Ch. 12 - What role does cAMP play in transcription of lac...Ch. 12 - How would a cap- mutation that produces an...
Ch. 12 - Explain the circumstances under which attenuation...Ch. 12 - Consider the transcription of genes of the...Ch. 12 - Describe the lytic and lysogenic life cycles of ...Ch. 12 - 12.14 Define antisense RNA, and describe how it...Ch. 12 - 12.15 Attenuation of trp operon transcription is...Ch. 12 - 12.16 In the lac operon, what are the likely...Ch. 12 - Identify which of the following lac operon haploid...Ch. 12 - Prob. 18PCh. 12 - 12.19 List possible genotypes for lac operon...Ch. 12 - Suppose each of the genotypes you listed in parts...Ch. 12 - 12.21 Four independent mutants (mutants A to D)...Ch. 12 - Suppose the lac operon partial diploid...Ch. 12 - What is a riboswitch? Describe the riboswitch...Ch. 12 - 12.24 A repressible operon system, like the trp...Ch. 12 - 12.25 What is the likely effect of each of the...Ch. 12 - 12.26 Suppose that base substitution mutations...Ch. 12 - 12.27 Two different mutations affect. Mutant...Ch. 12 - How would mutations that inactivate each of the...Ch. 12 - The bacterial insertion sequence IS 10 uses...Ch. 12 - For an E. coli strain with the lac operongenotype...Ch. 12 - 12.31 How could antisense RNA be used as an...Ch. 12 - 12.32 Section describes the function of tRNA...Ch. 12 - The following hypothetical genotypes have genes A,...Ch. 12 - 12.34 Northern blot analysis is performed on...Ch. 12 - Prob. 35PCh. 12 - Prob. 36PCh. 12 - 12.37 The electrophoresis gel shown in part (a) is...
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- The linear dsDNA genome of λ binds on the LamB receptor of E. Coli and conducts a normal lysogenic cycle. Exposure to stress will cause the excision of λ prophage from the E. Coli genome. The excised λ genome is then replicated, packaged, and released from the cell as mature λ phage particles and ready to infect other bacterial cells. Among λ phage particles,the transducing phage mediates a specific type of recombination. Understand this scenario and answer the following questions. 1. What are the basic requirements for the insertion of λ into the E. Coli genome? 2. What special features are found in the λ insertion site? 3. What type of recombination occurs with λ insertion in the E. Coli genome? 4. How you will differentiate λ transducing phage from normal λ phage? 5. What exclusive mechanism λ phage utilizes for recombination?arrow_forwardA researcher is studying the rII locus of phage T4. FourrII− strains are obtained: A, B, C, and D. In the first experiment, E. coli strain K(λ) is coinfected with two rII− strains simultaneously and the results are recorded. Infection with A and B phage = lysis occurs Infection with A and C phage = lysis occurs Infection with B and C phage = no lysis occurs Infection with B and D phage = no lysis occurs Infection with C and D phage = no lysis occurs In a second experiment, coinfections are performed first in E. coli strain B, then the progeny phage are used to infect E. coli strain K(λ). Progeny of A and B phage = plaques form Progeny of B and C phage = plaques form Progeny of C and D phage = plaques form Progeny of B and D phage = no plaques from Which conclusions are consistent with these data? Why? A) Strains A and B carry mutations in the same gene. B) Strains B and D both carry the same mutation. C) Strains B, C, and D carry mutations in the same gene. D)…arrow_forwardAlfred Hershey and Martha Chase performed experiments to show that A: the genetic material was DNA contained in the bacteriophage, which entered bacteria during phage infection. B:the genetic material was RNA contained in the bacteriophage, which entered bacteria during phage infection C:the genetic material was protein contained in the bacteriophage, which entered bacteria during phage infection D:none of these choices are correctHomework question i am lost onarrow_forward
- In a genetics lab, Kim and Maria infected a samplefrom an E. coli culture with a particular virulent bacteriophage. They noticed that most of the cells werelysed, but a few survived. The survival rate in theirsample was about 1 × 10−4. Kim was sure the bacteriophage induced the resistance in the cells, whileMaria thought that resistant mutants probably alreadyexisted in the sample of cells they used. Earlier, for adifferent experiment, they had spread a dilute suspension of E. coli onto solid medium in a large petri dish,and, after seeing that about 105colonies were growingup, they had replica-plated that plate onto three otherplates. Kim and Maria decide to use these plates totest their theories. They pipette a suspension of thebacteriophage onto each of the three replica plates.What should they see if Kim is right? What shouldthey see if Maria is right?arrow_forwardBacteria exposed to viruses incorporate sections of the virus’s DNA into the CRISPR array sequences in their genome. This mechanism allows bacteria to fight off the viruses, like an immune response: the information in CRISPR spacers served as “coordinates” for recognizing and cutting up invading DNA sequences. Describe what might happen under the conditions described after a bacteriophage infects a bacterial cell and releases its DNA into the bacterial cell. Explain why: 1. The invading phage DNA is recognized by the Cas proteins but not inserted into the CRISPR array region of the bacterial genome: The bacteria will be unable to elicit an immune response and will succumb to the phase infection 2. The cas genes on the bacterial genome contains a missense mutation that increases its cleavage/cut activityThe bacteria will elicit an immune response that will successfully fight the phage infectionarrow_forwardDescribe in general terms how bacteriophage lambda regulates the switch between lytic and lysogenic cyclesarrow_forward
- There can be more than one answerarrow_forwardwhen various strains of lambda phage are seeded on a lawn of e.coli, they can form clear or turbid plaques. Explain the difference between the two types of plaques. can all bacteriophage form clear and turbid plaques?arrow_forwardA researcher is studying the r// locus of phage T4. Three //-- strains are obtained: A, B, and C. E. coli strain K(A) is coinfected with two rl strains simultaneously and the results are recorded. Infection with A and B phage = lysis occurs Infection with A and C phage = lysis occurs Infection with B and C phage = no lysis occurs What can be inferred from this complementation test? Multiple Choice Strains A, B, and C have mutations in three different genes. Strains A and B have mutations in the same gene, and strain C has a mutation in a different gene. Strains A and C have mutations in the same gene, and strain B has a mutation in a different gene. Strains A, B, and C have mutations in the same gene. Strains B and C have mutations in the same gene, and strain A has a mutation in a different gene.arrow_forward
- For bacteria that are F+, Hfr, F', and F- answer the following. a. Describe the state of the F factor. b. Which of these cells are donors? Which is the recipient? c. Which of these donors can convert exconjugants to a donor state? d. Which of these donors can transfer a donor gene to exconjugants? e. Describe the results of conjugation (i.e., changes in the recipient and the exconjugant) that allow detection of the state of the F factor in a donor strain. f. Describe a "partial diploid" and how it originates.arrow_forwardYou are determined to produce an antiserum for the next pandemic that has hit the world. You have found a gene of interest in an animal in the rainforest which produces a unique protein that blocks the reproduction of the pathogen that now plagues the world. You wish to mass produce this protein using bacteria.arrow_forwardOrder the following experimental steps to identify auxotrophic mutants in Saccharomyces Cerevisiae that cannot sythesize their own Leucine. an option can be selected more than once. step 1: Step 2: Step 3: Step 4: Step 5: Step 6:arrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license