Chapter 12, Problem 23CQ

Summary Introduction

To determine: The K_M value of an enzyme with substrate concentration and reaction velocity.

Concept introduction: Enzymes are the biological protein catalysts that alter the speed of the reaction in a biological system. The chemical reactions that are catalyzed by these enzymes are studied under the enzyme kinetics. The reaction rate is measured based on the rate at which the substrate of the enzymes combine with the enzyme and form the products. K_M is the Michaelis-Menton constant. K_M value is not a direct measure of the affinity of the enzyme toward the substrate but the concentration of S, where the velocity at the maximum is half. V_max is the maximum velocity with which the enzyme catalyzes the reaction. 

$\text{The rate of enzyme reaction }\left(v_0\right)\text{ =}\frac{{\text{V}}_{\text{max}}\left[\text{S}\right]}{\left({\text{K}}_{\text{M}}\text{+}\left[\text{S}\right]\right)}$.