An experimental protocol that allows the identification and isolation of cDNA for hexokinase is to be proposed. Also, an experimental protocol to verify that the identified protein is hexokinase A is to be stated. Concept introduction: The strands of DNA molecule contain the complementary pairs of bases. One nucleotide sequence of one strand automatically provides the details required to form its partner. The adenine is complementary nucleotide for thymine and cytosine is the complementary nucleotide for guanine. A hexokinase A is an enzyme that attaches phosphoryl group to a hexose and results in the formation of hexose phosphate. It can transfer an inorganic phosphate group from ATP to a substrate molecule.

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Chapter 12, Problem 13P

Interpretation Introduction

Interpretation:

An experimental protocol that allows the identification and isolation ofcDNAfor hexokinase is to be proposed. Also, an experimental protocol to verify that the identified protein is hexokinase A is to be stated.

Concept introduction:

The strands of DNA molecule contain the complementary pairs of bases. One nucleotide sequence of one strand automatically provides the details required to form its partner. The adenine is complementary nucleotide for thymine and cytosine is the complementary nucleotide for guanine.

A hexokinase A is an enzyme that attaches phosphoryl group to a hexose and results in the formation of hexose phosphate. It can transfer an inorganic phosphate group from ATP to a substrate molecule.

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