Campbell Biology: Custom Edition
18th Edition
ISBN: 9781323717271
Author: Urry, Cain, Wasserman, Minorsky, Reece
Publisher: PEARSON C
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Textbook Question
Chapter 12, Problem 12.2CR
In which of the three phases of interphase and the stages of mitosis do chromosomes exist as Single DNA molecules?
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 12 Solutions
Campbell Biology: Custom Edition
Ch. 12.1 - How many chromosomes are drawn in each part of...Ch. 12.1 - WHAT IF? A chicken has 78 chromosomes in its...Ch. 12.2 - How many chromosomes are shown in the illustration...Ch. 12.2 - Compare cytokinesis in animal cells and plant...Ch. 12.2 - During which stages of the cell cycle does a...Ch. 12.2 - Compare the roles of tubulin and actin during...Ch. 12.2 - A kinetochore has been compared to a coupling...Ch. 12.2 - MAKE CONNECTIONS What other functions do actin...Ch. 12.3 - In Figure 12.14, why do the nuclei resulting from...Ch. 12.3 - How does MPF allow a cell to pass the G2 phase...
Ch. 12.3 - MAKE CONNECTIONS Explain how receptor tyrosine...Ch. 12 - Differentiate between these terms: chromosome,...Ch. 12 - In which of the three phases of interphase and the...Ch. 12 - Explain the significance of the G1, G2, and M...Ch. 12 - Through a microscope, you can see a cell plate...Ch. 12 - Vinblastine is a standard chemotherapeutic drug...Ch. 12 - One difference between cancer cells and normal...Ch. 12 - The decline of MPF activity at the end of mitosis...Ch. 12 - In the cells of some organisms, mitosis occurs...Ch. 12 - Which of the following does not occur during...Ch. 12 - Cell A has half as much DNA as cells B, C, and D...Ch. 12 - The drug cytochalasin B blocks the function of...Ch. 12 - VISUAL SKILLS The light micrograph shows dividing...Ch. 12 - DRAW IT Draw one eukaryotic chromosome as it would...Ch. 12 - EVOLUTION CONNECTION The result of mitosis is that...Ch. 12 - SCIENTIFIC INQUIRY Although both ends of a...Ch. 12 - WRITE ABOUT A THEME: INFORMATION The continuity of...Ch. 12 - SYNTHESIZE YOUR KNOWLEDGE For selected answers,...
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