Human Anatomy
6th Edition
ISBN: 9781260399820
Author: SALADIN, Kenneth
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 12, Problem 12.2.4AYLO
Summary Introduction
To analyze:
Deep lateral rotators of the hip region.
Introduction:
The lateral rotator is an aggregate of muscles found in the gluteal part of the body. The lateral rotator muscles are 4 in number, all the muscles help in lateral rotation of hip joint and the other body movements like abduction and extension of muscles.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 12 Solutions
Human Anatomy
Ch. 12.1 - Prob. 1AWYKCh. 12.1 - Prob. 2AWYKCh. 12.1 - Prob. 1BYGOCh. 12.1 - Prob. 2BYGOCh. 12.1 - Prob. 3BYGOCh. 12.1 - Prob. 4BYGOCh. 12.1 - Prob. 5BYGOCh. 12.1 - Prob. 6BYGOCh. 12.2 - Not everyone has the same muscles. From the...Ch. 12.2 - Prob. 7BYGO
Ch. 12.2 - Name the muscles that cross both the hip and knee...Ch. 12.2 - Prob. 9BYGOCh. 12.2 - Prob. 10BYGOCh. 12.3 - Prob. 11BYGOCh. 12.3 - Prob. 12BYGOCh. 12 - Prob. 12.1.1AYLOCh. 12 - Prob. 12.1.2AYLOCh. 12 - Prob. 12.1.3AYLOCh. 12 - Prob. 12.1.4AYLOCh. 12 - Prob. 12.1.5AYLOCh. 12 - Prob. 12.1.6AYLOCh. 12 - Prob. 12.1.7AYLOCh. 12 - Prob. 12.1.8AYLOCh. 12 - Prob. 12.1.9AYLOCh. 12 - Prob. 12.1.10AYLOCh. 12 - Prob. 12.1.11AYLOCh. 12 - Prob. 12.1.12AYLOCh. 12 - Prob. 12.1.13AYLOCh. 12 - Prob. 12.1.14AYLOCh. 12 - Prob. 12.1.15AYLOCh. 12 - Prob. 12.2.1AYLOCh. 12 - The three gluteal muscles-gluteus maximus, gluteus...Ch. 12 - The tensor fasciae latae of the hip and its...Ch. 12 - Prob. 12.2.4AYLOCh. 12 - Prob. 12.2.5AYLOCh. 12 - Prob. 12.2.6AYLOCh. 12 - Prob. 12.2.7AYLOCh. 12 - The hamstring muscles of the posterior compartment...Ch. 12 - Prob. 12.2.9AYLOCh. 12 - Prob. 12.2.10AYLOCh. 12 - Prob. 12.2.11AYLOCh. 12 - Prob. 12.2.12AYLOCh. 12 - Prob. 12.2.13AYLOCh. 12 - Prob. 12.2.14AYLOCh. 12 - Prob. 12.2.15AYLOCh. 12 - Prob. 12.3.1AYLOCh. 12 - Prob. 12.3.2AYLOCh. 12 - Prob. 1TYRCh. 12 - Prob. 2TYRCh. 12 - Prob. 3TYRCh. 12 - Prob. 4TYRCh. 12 - Which of these muscles does not contribute to the...Ch. 12 - Which of these actions is not performed by the...Ch. 12 - Prob. 7TYRCh. 12 - Prob. 8TYRCh. 12 - Both the gastrocnemius and ____________ muscles...Ch. 12 - Prob. 10TYRCh. 12 - Prob. 11TYRCh. 12 - Prob. 12TYRCh. 12 - Pronation of the forearm is achieved by two...Ch. 12 - Prob. 14TYRCh. 12 - Prob. 15TYRCh. 12 - Prob. 16TYRCh. 12 - Prob. 17TYRCh. 12 - Prob. 18TYRCh. 12 - Prob. 19TYRCh. 12 - Prob. 20TYRCh. 12 - Prob. 1BYMVCh. 12 - Prob. 2BYMVCh. 12 - State a meaning of each world element and give a...Ch. 12 - Prob. 4BYMVCh. 12 - Prob. 5BYMVCh. 12 - Prob. 6BYMVCh. 12 - State a meaning of each world element and give a...Ch. 12 - Prob. 8BYMVCh. 12 - Prob. 9BYMVCh. 12 - Prob. 10BYMVCh. 12 - Briefly explain why each of the following...Ch. 12 - Prob. 2WWWTSCh. 12 - Prob. 3WWWTSCh. 12 - Prob. 4WWWTSCh. 12 - Prob. 5WWWTSCh. 12 - Briefly explain why each of the following...Ch. 12 - Briefly explain why each of the following...Ch. 12 - Prob. 8WWWTSCh. 12 - Prob. 9WWWTSCh. 12 - Prob. 10WWWTSCh. 12 - Prob. 1TYCCh. 12 - Prob. 2TYCCh. 12 - Poorly conditioned middle-aged people may suffer a...Ch. 12 - Women who habitually wear high heels may suffer...Ch. 12 - A student moving out of a dormitory crouches in...
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