Foundations in Microbiology
10th Edition
ISBN: 9781259705212
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
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Chapter 11.L1, Problem 1WC
Summary Introduction
Introduction:
In the phrase “prior to vaccination, the patient’s skin was sterilized with alcohol” use of the word “sterilize” is incorrect. Instead of it the word degermation should be used. Sterilization is used to define the process of removal of all microbes and spores on inanimate objects and surfaces.
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 11 Solutions
Foundations in Microbiology
Ch. 11.1 - Prob. 1ELOCh. 11.1 - Prob. 2ELOCh. 11.1 - 3. Define and differentiate among the major terms...Ch. 11.1 - 4. Characterize the parameters of microbial death,...Ch. 11.1 - 5. Summarize what factors influence the...Ch. 11.1 - 6. Identify the targets of antimicrobial control...Ch. 11.1 - Prob. 1CYPCh. 11.1 - Prob. 2CYPCh. 11.1 - 3. Contrast various microbes and rate their...Ch. 11.1 - 4. Explain how the types and numbers of...
Ch. 11.1 - Prob. 5CYPCh. 11.1 - Prob. 6CYPCh. 11.1 - Prob. 7CYPCh. 11.1 - Prob. 8CYPCh. 11.2 - Prob. 7ELOCh. 11.2 - Prob. 8ELOCh. 11.2 - Prob. 9ELOCh. 11.2 - 10. Differentiate between thermal death point and...Ch. 11.2 - 11. Describe several moist heat methods and their...Ch. 11.2 - Prob. 12ELOCh. 11.2 - Prob. 13ELOCh. 11.2 - 9. What happens to microbes that encounter...Ch. 11.2 - 10. Summarize the nature, mode of action, and...Ch. 11.2 - 11. Explain the concepts of TDT and TDP, using...Ch. 11.2 - Prob. 12CYPCh. 11.2 - 13. How can the temperature of steam be raised...Ch. 11.2 - Prob. 14CYPCh. 11.2 - Prob. 15CYPCh. 11.2 - 16. Explain why desiccation and cold are not...Ch. 11.3 - Prob. 14ELOCh. 11.3 - 15. Differentiate between ionizing and nonionizing...Ch. 11.3 - Prob. 16ELOCh. 11.3 - Prob. 17ELOCh. 11.3 - Prob. 17CYPCh. 11.3 - Prob. 18CYPCh. 11.3 - 19. What are some advantages and disadvantages of...Ch. 11.3 - Prob. 20CYPCh. 11.3 - Prob. 21CYPCh. 11.4 - Prob. 18ELOCh. 11.4 - 19. Explain the desirable features of...Ch. 11.4 - 20. Describe the types of halogens, their modes of...Ch. 11.4 - 21. Identify the characteristics of phenolic...Ch. 11.4 - 22. Describe the characteristics of oxidizing...Ch. 11.4 - Prob. 23ELOCh. 11.4 - 24. Explain how detergents, soaps, and heavy...Ch. 11.4 - 22. Describe situations that require high-level...Ch. 11.4 - 23. What is the difference between a tincture and...Ch. 11.4 - 24. Define sterilant, and name the principal...Ch. 11.4 - 25. Summarize the chief forms and uses of chlorine...Ch. 11.4 - 26. What are the superior characteristics of...Ch. 11.4 - 27. What are the modes of action of alcohols and...Ch. 11.4 - 28. Why is hydrogen peroxide solution so effective...Ch. 11.4 - 29. Give the uses and disadvantages of the heavy...Ch. 11.4 - 30. What does it mean to say that a chemical has...Ch. 11.L1 - Prob. 1MCQCh. 11.L1 - Prob. 2MCQCh. 11.L1 - Prob. 3MCQCh. 11.L1 - Prob. 4MCQCh. 11.L1 - Prob. 5MCQCh. 11.L1 - Prob. 6MCQCh. 11.L1 - 7. The primary action of ______ heat is to ______....Ch. 11.L1 - Prob. 8MCQCh. 11.L1 - 9. Microbe(s) that is/are the target(s) of...Ch. 11.L1 - 10. Ionizing radiation like _________ removes...Ch. 11.L1 - Prob. 11MCQCh. 11.L1 - Prob. 12MCQCh. 11.L1 - Prob. 13MCQCh. 11.L1 - 14. A chemical with sporicidal properties is a....Ch. 11.L1 - 15. Silver sulfadiazine is used a. in antisepsis...Ch. 11.L1 - Prob. 16MCQCh. 11.L1 - Prob. 17MCQCh. 11.L1 - 1. How would one best describe the state of being...Ch. 11.L1 - Prob. 2CSRCh. 11.L1 - Prob. 3CSRCh. 11.L1 - Prob. 1WCCh. 11.L1 - Prob. 2WCCh. 11.L1 - Prob. 3WCCh. 11.L1 - 4. Think of three situations in which the same...Ch. 11.L1 - 5. Explain what features of endospores make them...Ch. 11.L1 - 6. Explain some of the problems involved in...Ch. 11.L1 - 7. The shelf life and keeping qualities of fruit...Ch. 11.L2 - 1. For each item on the following list, propose a...Ch. 11.L2 - Prob. 2CTCh. 11.L2 - 3. It may seem contradictory that lyophilization...Ch. 11.L2 - 4. A supermarket/drugstore assignment: Look at the...Ch. 11.L2 - 5. Devise an experiment that will differentiate...Ch. 11.L2 - 6. There is quite a bit of concern that chlorine...Ch. 11.L2 - 7. Was the source patient in the case study most...Ch. 11.L2 - 8. Explain why surfactant chemicals are more...Ch. 11.L2 - From chapter 2, figure 2.20. Study this...Ch. 11.L2 - 2. Explain what is happening with this graph that...
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