Biology
Biology
4th Edition
ISBN: 9781259188121
Author: Peter Stiling, Robert Brooker, Linda Graham, Eric Widmaier
Publisher: McGraw-Hill Education
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Chapter 11.4, Problem 3CC
Summary Introduction

To determine: The movement of primase in the lagging strand in figure 11.19.

Introduction: The process of formation of daughter strands from DNA strands is termed as replication of DNA. The enzyme RNA primer initiates the synthesis of daughter DNA strands from the template strand. The RNA primer is synthesized by an enzyme known as DNA primase. Two strands are synthesized by this enzyme. One is the leading strand while the other is the lagging strand.

Summary Introduction

To determine: Whether primase moves from left to right or from right to left.

Introduction: The process of formation of daughter strands from DNA strands is termed as replication of DNA. The enzyme RNA primer initiates the synthesis of daughter DNA strands from the template strand. The RNA primer is synthesized by an enzyme known as DNA primase. Two strands are synthesized by this enzyme. One is the leading strand while the other is the lagging strand.

Summary Introduction

To determine: The way in which primase must move after it is done making a primer and has to start making the next primer at a new location.

Introduction: The process of formation of daughter strands from DNA strands is termed as replication of DNA. The enzyme RNA primer initiates the synthesis of daughter DNA strands from the template strand. The RNA primer is synthesized by an enzyme known as DNA primase. Two strands are synthesized by this enzyme. One is the leading strand while the other is the lagging strand.

Summary Introduction

To determine: Whether primase has to hop from left to right or right to left direction.

Introduction: The process of formation of daughter strands from DNA strands is termed as replication of DNA. The enzyme RNA primer initiates the synthesis of daughter DNA strands from the template strand. The RNA primer is synthesized by an enzyme known as DNA primase. Two strands are synthesized by this enzyme. One is the leading strand while the other is the lagging strand.

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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?   The cell membranes are more resistant than normal.   An isotonic solution had been added instead of water.   A solution of 0.1 M NaCl had been added instead of water.   Not enough water had been added to the red blood cell pellet.   The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
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