Microbiology with Diseases by Body System (5th Edition)
5th Edition
ISBN: 9780134477206
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 11, Problem 7SA
Summary Introduction
To answer:
In what way, the Agrobacterium is used in recombinant DNA technology.
Introduction:
Recombinant DNA technology plays an important role in the field of industry, science, agriculture, and medicine. They generate new genetic combinations by joining two different DNAs (species).
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Describe how would you determine the following using a real-time PCR instrument.
A) The quantity and quality of a DNA sample.
B) Whether an individual had cystic fibrosis from a genomic DNA sample.
C) If several bacterial pathogens were present in DNA extracted from a food sample.
Why are bacteria excellent hosts for recombinant DNA experiments?
Why is it important to use a hyperthermophilic DNA polymerase in PCR?
a) Because only hyperthermophiles have DNA polymerases.
b) Because hyperthermophilic DNA polymerase is able to resist the saline reaction conditions.
c) Because hyperthermophilic DNA polymerase is faster than other polymerases.
d) Because hyperthermophilic DNA polymerase is able to resist denaturation at 95℃.
Chapter 11 Solutions
Microbiology with Diseases by Body System (5th Edition)
Ch. 11 - Prob. 1TMWCh. 11 - Why are taxonomic names and categories in our...Ch. 11 - Why is it that archaea dont seem to cause disease...Ch. 11 - Prob. 1EDCSCh. 11 - Why are bacteria all classified in the same domain...Ch. 11 - Modified True/False 1. ____ All prokaryotes...Ch. 11 - Prob. 2MTFCh. 11 - Modified True/False 3. __________ If you were to...Ch. 11 - Prob. 4MTFCh. 11 - Prob. 5MTF
Ch. 11 - Modified True/False 6. __________ Halophiles...Ch. 11 - Prob. 7MTFCh. 11 - Prob. 8MTFCh. 11 - Modified True/False 9. A giant bacterium that is...Ch. 11 - Prob. 10MTFCh. 11 - Prob. 1MCCh. 11 - Prob. 2MCCh. 11 - Prob. 3MCCh. 11 - Endospores _____. a. remain alive for decades b....Ch. 11 - Prob. 5MCCh. 11 - Prob. 6MCCh. 11 - Prob. 7MCCh. 11 - Prob. 8MCCh. 11 - Prob. 9MCCh. 11 - The presence of mycolic acid in the cell wall...Ch. 11 - Match the bacterium on the left with the term with...Ch. 11 - Whereas the first edition of Bergeys Manual relied...Ch. 11 - What are extremophiles? Describe two kinds, and...Ch. 11 - Name and describe three types of bacteria...Ch. 11 - Prob. 4SACh. 11 - Prob. 5SACh. 11 - A student was memorizing the arrangements of...Ch. 11 - Prob. 7SACh. 11 - Name and describe five distinct classes of phylum...Ch. 11 - Prob. 9SACh. 11 - Prob. 1VICh. 11 - Prob. 2VICh. 11 - A microbiology student described deeply branching...Ch. 11 - Prob. 2CTCh. 11 - Prob. 3CTCh. 11 - Prob. 4CTCh. 11 - Louis Pasteur said, The role of the infinitely...Ch. 11 - Prob. 6CTCh. 11 - A scientist who discovers a prokaryote living in a...Ch. 11 - Contrast the processes of nitrogen fixation and...Ch. 11 - Prob. 9CTCh. 11 - How do scientists know there are millions of...Ch. 11 - Why might soil-dwelling organisms such as...Ch. 11 - Using the following terms, fill in the following...
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- What makes the species of Agrobacterium ideal for genetic engineering? Describe its characteristics and its role in producing transgenic plants.arrow_forwardWhat are plasmids? What is the importance of plasmids for the recombinant DNA technology?arrow_forwardTransfection efficiency refers to a) total number of transfected cells per dish O b) total number of transfected cells per field of view total amount of plasmid DNA added per sample d) number of cells per 100 that have taken up plasmid DNAarrow_forward
- What is recombinant DNA technology ?arrow_forwardWhy is Agrobacteriummediated genetic engineering transformation inplants considered as natural genetic engineering?arrow_forwardWhich is used for cloning eukaryotic genes but not prokaryotic genes?a) Restriction enzymesb) DNA ligasec) Reverse transcriptased) Vectore) Selectable markerarrow_forward
- An example of bioremediation is A) the use of prokaryotes to treat sewage or clean up oil spills. B) the production of antibiotics by cultured prokaryotes. C) the application of bacteria to produce transgenic plants.arrow_forwardHow are we to know which of the bacteria have taken up the plasmid?arrow_forwardSequence of which of the following cannot be determined using the Maxam Gilbert method?a) Bacteriab) Plantsc) Bacteriophage T7d) Plasmidarrow_forward
- 1. What is a plasmid? A) An organelle found in plants that conducts photosynthesis. B) Circular extrachromosomal DNA. C) An essential part of the bacterial chromosome. 2. What is the role of the NaOH in plasmid isolation? A) It increases the pH. B) It denatures the DNA. C) Both of the above. 3. In which direction will the DNA migrate in an agarose gel? A) To the positive pole. B) To the negative pole. C) That depends on the sequence of the DNA. 4. What is the function of SDS? A) It denatures proteins. B) It disintegrates the cell membrane. C) Both of the above. 5. To which species group does E. coli belong to? A) Bacteria, B) Fungi. C) Archaea.arrow_forwardWhat is Recombinant DNA technology ? Who created this ?arrow_forward17) What gives Aspergillus sydowii colonies their hairy appearance? a) Hyphae () b) Stipes c) Growing media ) d) Ascocarpoda Jack has designed one primer which is complementary to the DNA of E. coli. He then used that primer for a PCR reaction using DNA isolated from a mixture of bacteria. He got no PC reaction product and hence concluded that there is absolutely no E. coli in the bacteria mixture. His conclusion is .... () True ) Falsearrow_forward
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