EBK PRINCIPLES OF BIOLOGY
EBK PRINCIPLES OF BIOLOGY
2nd Edition
ISBN: 8220103675338
Author: BROOKER
Publisher: YUZU
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Chapter 11, Problem 3CCQ
Summary Introduction

To analyze:

The ways by which the structure of HOTAIR allows it to carry out its function.

Introduction:

The non-coding RNAs bind with DNA or RNA because of complementary base pairing. This allows the non-coding RNAs to affect the processes such as DNA replication, transcription and translation.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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