Concepts of Genetics (12th Edition)
12th Edition
ISBN: 9780134604718
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino, Darrell Killian
Publisher: PEARSON
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Textbook Question
Chapter 11, Problem 1NST
In the Meselson-Stahl experiment, which of the three modes of replication could be ruled out after one round of replication? after two rounds?
Expert Solution
Summary Introduction
To determine: The reason that in the Meselson– Stahl experiment among the three modes of replication, the mode of replication that could be ruled out after oneround of replication.
Introduction: Messelson and Stahl experiment explained that semiconservative mode of replication used by bacterial cells to produce new DNA molecules.
Explanation of Solution
Messelson grew the E. coli cells for many generations in a medium containing 15NH4Cl. These cells then transferred to 14NH4Cl. After one round of replication, the conservative mechanism of DNA replication was ruled out as the intermediate density of DNA band found in the solution. DNA undergoes the semiconservative mode of replication.
Conclusion
Thus, the conservative mode of replication can be ruled out after one round of replication in case of Meselshon Stahl experiment.
Expert Solution
Summary Introduction
To determine: The reason that in Meselson– Stahl experiment among the three modes of replication the mode of replication that could be ruled out after the second round of replication.
Introduction: Messelson and Stahlstudied the modes of DNA replication in bacteria by using nitrogen and its isotope in the medium. Bands of DNA replication was studied after each round of replication.
Explanation of Solution
Messelson grew the bacterial DNA in N15 and N14 media. After one round of replication in14N media, the band of the intermediate density of DNA was found.
After the second round of replication in14N media, the dispersive model of DNA replication was ruled out. The density of the DNA after the second round of replication was between the intermediate (both dark and light band) and “light” densities of DNA.
Conclusion
Thus, the dispersive model of DNA replication was ruled out after the second round of replication.
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Students have asked these similar questions
In the Meselson Stahl experiment differentiating the possible modes of replication, how many rounds of replication were needed to determine that the following modes of replication were not used in E. coli?
two rounds of replication for conservative; two rounds of replication for dispersive
one round of replication for conservative; two rounds of replication for dispersive
two rounds of replication for conservative; one round of replication for dispersive
one round of replication for conservative; one round of replication for dispersive
none of the above is correct
After two generations of replication in the Meselson and Stahl
experiment, what was the composition of the two bands?
One band was all 14N and one band was half 14N and half 15N.
One band was all 15N and one band was half 14N and half 15N.
One band was all 14N and one band was all 15N.
One band was all 15N and one band was one quarter 14N and three
quarters 15N.
One band was all 14N and one band was one quarter 14N and three
quarters 15N.
How many bands of DNA would be expected in Meselson and Stahl’s experiment after two rounds of conservative replication?
Chapter 11 Solutions
Concepts of Genetics (12th Edition)
Ch. 11 - In the Meselson-Stahl experiment, which of the...Ch. 11 - An alien organism was investigated. When DNA...Ch. 11 - Why might mutations in genes encoding telomerase...Ch. 11 - Although the brother is an immunologically matched...Ch. 11 - Prob. 3CSCh. 11 - HOW DOWE KNOW? In this chapter, we focused on how...Ch. 11 - CONCEPT QUESTION Review the Chapter Concepts list...Ch. 11 - Compare conservative, semiconservative, and...Ch. 11 - Describe the role of 15N in the MeselsonStahl...Ch. 11 - Predict the results of the experiment by Taylor,...
Ch. 11 - What are the requirements for in vitro synthesis...Ch. 11 - In Kornbergs initial experiments, it was rumored...Ch. 11 - How did Kornberg assess the fidelity of DNA...Ch. 11 - Which characteristics of DNA polymerase I raised...Ch. 11 - Kornberg showed that nucleotides are added to the...Ch. 11 - What was the significance of the polA1 mutation?Ch. 11 - Summarize and compare the properties of DNA...Ch. 11 - List and describe the function of the ten subunits...Ch. 11 - Distinguish between (a) unidirectional and...Ch. 11 - List the proteins that unwind DNA during in vivo...Ch. 11 - Define and indicate the significance of (a)...Ch. 11 - Outline the current model for DNA synthesis.Ch. 11 - Why is DNA synthesis expected to be more complex...Ch. 11 - Suppose that E. coli synthesizes DNA at a rate of...Ch. 11 - Several temperature-sensitive mutant strains of E....Ch. 11 - While many commonly used antibiotics interfere...Ch. 11 - Describe the end-replication problem in...Ch. 11 - Many of the gene products involved in DNA...Ch. 11 - In 1994, telomerase activity was discovered in...Ch. 11 - The genome of D. melanogaster consists of...Ch. 11 - Prob. 26ESPCh. 11 - DNA polymerases in all organisms add only 5...Ch. 11 - Assume that the sequence of bases shown below is...Ch. 11 - Reiji and Tuneko Okazaki conducted a now classic...Ch. 11 - Consider the drawing of a dinucleotide below. (a)...Ch. 11 - To gauge the fidelity of DNA synthesis, Arthur...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- a) "Out of three E.coli DNA polymerases, DNA polymerases 3 has a high processivity and rate of polymerization and therefore better suited for replication of the genome" What is meant by processivity? how does the DNA polymerase 3 maintain high processivity? b) What is a replication fork ?. Give the protein/enzymes of a replication fork and describe their function?arrow_forwardA temperature sensitive mutant is isolated where at the non-permissive temperature replication stops relatively slowly and when the DNA is analyzed it appears to have extra twists ahead of the replication forks. What protein is most likely mutated? a)DnaA b)DNA polymerase I c)DNA gyrase d) DNA polymerase IIIarrow_forwardWhat is meant by semi conservative replication? How did Meselson and Stahl prove it experimentally?arrow_forward
- he Meselson and Stahl experiment provided conclusive evidence for the semiconservative replication of DNA in E. coli. What pattern of bands would they have observed in a CsCl gradient after one generation if replication was conservative? a heavy, a light and an intermediate band one heavy and one light band (no intermediate) one intermediate band one light band one heavy bandarrow_forwardWhat are the three models of DNA replication? With the aid of illustrations, show how the Meselson Stahl experiment come to the conclusion of one model of DNA replication. Is DNA replication bidirectional? How did you arrive at this conclusion? Explain the bacterial replication model that supports this conclusion.arrow_forwardIf the sequence 5′-AACGC-3′ were damaged by reactive oxygen species, what would be the most prevalent product, and what would be the result of replication? (Note: show both strands after replication)arrow_forward
- In terms of the new DNA strands that are generated, what are the differences between replication and conventional polymerase chain reaction?arrow_forwardWhich of the following statements about DNA polymerase I are true? 1) It requires both template DNA and a primer. 2) It can copy and join the ends of single-stranded circular DNA. 3) It uses only DNA as a template primer. 4) With respect to linear DNA, it copies only the 5′ end and then dissociates. a) Only statements 1 and 3 are true. b) Only statements 1 and 4 are true. c) Only statements 1, 2, and 4 are true. d) All of the listed statements are true.arrow_forwardWhich of the following enzymes remove supercoiling in replicating DNA ahead of the replication fork?a) DNA polymerasesb) Helicasesc) Primasesd) Topoisomerasesarrow_forward
- What is the difference between semi-conservative replication and dispersive replication?arrow_forwardThe Meselson-Stahl experiment provided strong evidence that DNA replication was conservative, by alternately growing bacteria in medium with heavy 15N and light 14N. If DNA replication were dispersive, what result would Meselson and Stahl have observed after the first round of DNA replication in light nitrogen? Group of answer choices Two bands, one at the location for pure 15N and one at the location for pure 14N. One band, located half way between the locations for pure 15N and pure 14N. Two bands, one at the location for pure 15N and one located halfway between the locations for pure 15N and pure 14N. None of these Three bands, one at the location for pure 15N, one at the location for pure 14N, and one at a location halfway between.arrow_forwardIn the gel electrophoresis: the mutant would just run the same as the open circle DNA if it simply weren't able to close its circle. The mutant contains heavier than the single-stranded DNA because what kind of structure is it forming during replication? Wild-type phage DNA does not ever form a double-strand. What happens under denaturing conditions to that structure that helps explain that lane's smudge?In the sucrose centrifuging: what data from the gel electrophoresis support the conclusion?In the electrophoresis/Southern blot: which end of the DNA is the 1100 bp fragment, which end does the kinase ONLY work on?arrow_forward
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