Connect Online Access for Saladin Human Anatomy
11th Edition
ISBN: 9781260399738
Author: SALADIN, Kenneth
Publisher: Mcgraw-hill Higher Education (us)
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Chapter 11, Problem 11.4.6AYLO
Three muscles of the cheek, chin, and anterior neck-the mentalis, buccinator, and platysma (table 11.2)
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 11 Solutions
Connect Online Access for Saladin Human Anatomy
Ch. 11.1 - Name and define each connective tissue of a muscle...Ch. 11.1 - Describe how the fascicles of a muscle are...Ch. 11.1 - Prob. 3BYGOCh. 11.1 - Prob. 4BYGOCh. 11.1 - Prob. 5BYGOCh. 11.1 - Prob. 6BYGOCh. 11.2 - Prob. 1AWYKCh. 11.2 - Prob. 7BYGOCh. 11.2 - Prob. 8BYGOCh. 11.2 - Prob. 9BYGO
Ch. 11.3 - What is meant by the innervation of a muscle? Why...Ch. 11.3 - Prob. 11BYGOCh. 11.4 - Of the muscles you have studied so far, name three...Ch. 11.4 - Name two muscles that elevate the upper lip and...Ch. 11.4 - Name the four paired muscles of mastication and...Ch. 11.4 - Distinguish between the functions of the...Ch. 11.4 - List the prime movers of neck extension and...Ch. 11.5 - What muscles are eaten as “spare ribs”? What is...Ch. 11.5 - Prob. 16BYGOCh. 11.5 - Prob. 17BYGOCh. 11.5 - Name a major superficial muscle and two major deep...Ch. 11.5 - Define perineum, urogenital triangle, and anal...Ch. 11.5 - Name one muscle in the superficial perineal space,...Ch. 11 - Prob. 11.1.1AYLOCh. 11 - Prob. 11.1.2AYLOCh. 11 - Prob. 11.1.3AYLOCh. 11 - Prob. 11.1.4AYLOCh. 11 - Prob. 11.1.5AYLOCh. 11 - Prob. 11.1.6AYLOCh. 11 - Prob. 11.1.7AYLOCh. 11 - Prob. 11.1.8AYLOCh. 11 - Prob. 11.1.9AYLOCh. 11 - Prob. 11.2.1AYLOCh. 11 - Prob. 11.2.2AYLOCh. 11 - The three basic components of a lever and how...Ch. 11 - Why a single musculoskeletal lever cannot produce...Ch. 11 - Prob. 11.2.5AYLOCh. 11 - Prob. 11.3.1AYLOCh. 11 - Prob. 11.3.2AYLOCh. 11 - Prob. 11.4.1AYLOCh. 11 - Prob. 11.4.2AYLOCh. 11 - Prob. 11.4.3AYLOCh. 11 - Prob. 11.4.4AYLOCh. 11 - Prob. 11.4.5AYLOCh. 11 - Three muscles of the cheek, chin, and anterior...Ch. 11 - The difference between the intrinsic and extrinsic...Ch. 11 - Four extrinsic tongue muscles-the genioglossus,...Ch. 11 - Prob. 11.4.9AYLOCh. 11 - Prob. 11.4.10AYLOCh. 11 - Prob. 11.4.11AYLOCh. 11 - Four muscles of the infrahyoid group-the omohyoid,...Ch. 11 - Prob. 11.4.13AYLOCh. 11 - Prob. 11.4.14AYLOCh. 11 - Prob. 11.4.15AYLOCh. 11 - Prob. 11.4.16AYLOCh. 11 - The diaphragm and the three layers of intercostal...Ch. 11 - Prob. 11.5.2AYLOCh. 11 - Prob. 11.5.3AYLOCh. 11 - Prob. 11.5.4AYLOCh. 11 - Prob. 11.5.5AYLOCh. 11 - Prob. 11.5.6AYLOCh. 11 - Prob. 11.5.7AYLOCh. 11 - The boundaries of the perineum; its two triangles;...Ch. 11 - Prob. 11.5.9AYLOCh. 11 - Prob. 11.5.10AYLOCh. 11 - Prob. 11.5.11AYLOCh. 11 - Prob. 11.5.12AYLOCh. 11 - Prob. 1TYRCh. 11 - Prob. 2TYRCh. 11 - Which of these is not a suprahyoid muscle?...Ch. 11 - Prob. 4TYRCh. 11 - Prob. 5TYRCh. 11 - Prob. 6TYRCh. 11 - Prob. 7TYRCh. 11 - Prob. 8TYRCh. 11 - A muscle that aids in chewing without moving the...Ch. 11 - Prob. 10TYRCh. 11 - Prob. 11TYRCh. 11 - Prob. 12TYRCh. 11 - Prob. 13TYRCh. 11 - Prob. 14TYRCh. 11 - Prob. 15TYRCh. 11 - Prob. 16TYRCh. 11 - Prob. 17TYRCh. 11 - Prob. 18TYRCh. 11 - Prob. 19TYRCh. 11 - Prob. 20TYRCh. 11 - Prob. 1BYMVCh. 11 - Prob. 2BYMVCh. 11 - Prob. 3BYMVCh. 11 - Prob. 4BYMVCh. 11 - Prob. 5BYMVCh. 11 - Prob. 6BYMVCh. 11 - Prob. 7BYMVCh. 11 - State a meaning of each word element and give a...Ch. 11 - Prob. 9BYMVCh. 11 - Prob. 10BYMVCh. 11 - Prob. 1WWWTSCh. 11 - Prob. 2WWWTSCh. 11 - Briefly explain why each of the following...Ch. 11 - Prob. 4WWWTSCh. 11 - Prob. 5WWWTSCh. 11 - Prob. 6WWWTSCh. 11 - Prob. 7WWWTSCh. 11 - Prob. 8WWWTSCh. 11 - Prob. 9WWWTSCh. 11 - Prob. 10WWWTSCh. 11 - Name one antagonist of each of the following...Ch. 11 - Name one synergist of each of the following...Ch. 11 - Prob. 3TYCCh. 11 - Remova of cancerous lymph nodes from the neck...Ch. 11 - Prob. 5TYC
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