Anatomy & Physiology: The Unity of Form and Function
8th Edition
ISBN: 9781259277726
Author: Kenneth S. Saladin Dr.
Publisher: McGraw-Hill Education
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Chapter 11, Problem 10BYGO
Summary Introduction
Introduction:
The impulses from one neuron to neuron are transmitted by the aid of neurotransmitter chemicals and
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a) The amino acid sequence of the alpha chain terminates with a *. What does this symbol mean?
b) In your own words describe the function of the signal peptide and its final fate in post transcriptional modification.
c)How many amino acids form the precursor of insulin (preproinsulin)?
d) Since the C-peptide is cut out of proinsulin to create the final mature insulin (B-chain and A-chain) what role do you think the C-peptide might play in the biosynthesis of the mature insulin protein?
a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 11 Solutions
Anatomy & Physiology: The Unity of Form and Function
Ch. 11.1 - Five physiological properties of all muscular...Ch. 11.1 - Prob. 2AYLOCh. 11.1 - Prob. 3AYLOCh. 11.1 - Prob. 4AYLOCh. 11.1 - Prob. 2BYGOCh. 11.1 - Name and define the three layers of collagenous...Ch. 11.2 - Prob. 1AYLOCh. 11.2 - Prob. 2AYLOCh. 11.2 - Prob. 3AYLOCh. 11.2 - Prob. 4AYLO
Ch. 11.2 - Prob. 5AYLOCh. 11.2 - Prob. 6AYLOCh. 11.2 - Prob. 7AYLOCh. 11.2 - Prob. 8AYLOCh. 11.2 - Prob. 9AYLOCh. 11.2 - Prob. 10AYLOCh. 11.3 - Motor units; the meanings of large and small motor...Ch. 11.3 - Prob. 2AYLOCh. 11.3 - Prob. 3AYLOCh. 11.3 - Prob. 4AYLOCh. 11.3 - Prob. 5AYLOCh. 11.3 - How an action potential differs from the RMP, and...Ch. 11.4 - Prob. 1AYLOCh. 11.4 - Prob. 2AYLOCh. 11.4 - Prob. 3AYLOCh. 11.4 - Muscle relaxation; how the cessation of the nerve...Ch. 11.4 - Prob. 5AYLOCh. 11.4 - Prob. 6AYLOCh. 11.4 - Prob. 14BYGOCh. 11.5 - Prob. 1AYLOCh. 11.5 - The phases of a muscle twitchCh. 11.5 - Prob. 3AYLOCh. 11.5 - How recruitment and tetanus are produced and how...Ch. 11.5 - Prob. 5AYLOCh. 11.5 - Prob. 18BYGOCh. 11.6 - Prob. 1AYLOCh. 11.6 - Prob. 2AYLOCh. 11.6 - The use of myoglobin and aerobic respiration to...Ch. 11.6 - Prob. 4AYLOCh. 11.6 - How anaerobic fermentation generates ATP after the...Ch. 11.6 - Why a muscle is able to switch back to aerobic...Ch. 11.6 - Prob. 7AYLOCh. 11.6 - Vo2max, it partially determines ones ability to...Ch. 11.6 - Prob. 9AYLOCh. 11.6 - Differences between slow oxidative and fast...Ch. 11.6 - Prob. 11AYLOCh. 11.6 - Examples of resistance exercise and endurance...Ch. 11.7 - Prob. 1AYLOCh. 11.7 - Structural differences between cardiomyocytes and...Ch. 11.7 - Prob. 3AYLOCh. 11.7 - Prob. 4AYLOCh. 11.7 - Prob. 5AYLOCh. 11.7 - Prob. 6AYLOCh. 11.7 - Prob. 7AYLOCh. 11.7 - Prob. 8AYLOCh. 11.7 - Prob. 9AYLOCh. 11.7 - Prob. 10AYLOCh. 11.7 - Prob. 11AYLOCh. 11.7 - Prob. 12AYLOCh. 11.7 - Prob. 13AYLOCh. 11.7 - Prob. 14AYLOCh. 11.7 - The role of smooth muscle in peristalsisCh. 11.7 - Prob. 16AYLOCh. 11.7 - Prob. 26BYGOCh. 11.7 - Prob. 28BYGOCh. 11 - Prob. 1TYRCh. 11 - Prob. 2TYRCh. 11 - Prob. 3TYRCh. 11 - Prob. 4TYRCh. 11 - Prob. 5TYRCh. 11 - Prob. 6TYRCh. 11 - ACh receptors are found mainly in a. synaptic...Ch. 11 - Prob. 8TYRCh. 11 - Prob. 9TYRCh. 11 - Slow oxidative fibers have all of the following...Ch. 11 - Prob. 11TYRCh. 11 - Prob. 12TYRCh. 11 - Parts of the sarcoplasmic reticulum called ______...Ch. 11 - Prob. 14TYRCh. 11 - Prob. 15TYRCh. 11 - Prob. 16TYRCh. 11 - Prob. 17TYRCh. 11 - Prob. 18TYRCh. 11 - A state of continual partial muscle contraction is...Ch. 11 - Prob. 20TYRCh. 11 - Prob. 1BYMVCh. 11 - Prob. 2BYMVCh. 11 - dys-Ch. 11 - iso-Ch. 11 - Prob. 5BYMVCh. 11 - Prob. 6BYMVCh. 11 - Prob. 7BYMVCh. 11 - temporo-Ch. 11 - Prob. 9BYMVCh. 11 - Prob. 10BYMVCh. 11 - Prob. 1WWTSCh. 11 - Prob. 2WWTSCh. 11 - Prob. 3WWTSCh. 11 - Prob. 4WWTSCh. 11 - Thin filaments shorten when a muscle contracts.Ch. 11 - Smooth muscle lacks striations because it does not...Ch. 11 - Prob. 7WWTSCh. 11 - Prob. 8WWTSCh. 11 - Prob. 9WWTSCh. 11 - Prob. 10WWTSCh. 11 - Prob. 1TYCCh. 11 - Prob. 2TYCCh. 11 - Why would skeletal muscle be unsuitable for the...Ch. 11 - As skeletal muscle contracts, one or more bands of...Ch. 11 - Prob. 5TYCCh. 11 - Prob. 1BYGOCh. 11 - Prob. 2BYGOCh. 11 - Prob. 3BYGOCh. 11 - 4. What special terms are given to the plasma...Ch. 11 - Prob. 5BYGOCh. 11 - 6. List five proteins of the myofilaments and...Ch. 11 - Prob. 7BYGOCh. 11 - Prob. 8BYGOCh. 11 - Prob. 9BYGOCh. 11 - Prob. 10BYGOCh. 11 - Prob. 11BYGOCh. 11 - Prob. 12BYGOCh. 11 - Prob. 13BYGOCh. 11 - Prob. 14BYGOCh. 11 - Prob. 15BYGOCh. 11 - Prob. 16BYGOCh. 11 - Prob. 17BYGOCh. 11 - Prob. 18BYGOCh. 11 - Prob. 19BYGOCh. 11 - Prob. 20BYGOCh. 11 - Prob. 21BYGOCh. 11 - Prob. 22BYGOCh. 11 - Prob. 23BYGOCh. 11 - Prob. 24BYGOCh. 11 - Prob. 25BYGOCh. 11 - Prob. 26BYGOCh. 11 - Prob. 27BYGOCh. 11 - Prob. 28BYGOCh. 11 - Prob. 29BYGOCh. 11 - Prob. 30BYGO
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- a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates? b) Predict the growth you would expect to see on each of the following plates: – DNA ___________________________________________________________ – DNA/+Amp ______________________________________________________ +DNA/+Amp ______________________________________________________ +DNA/+Amp/+IPTG _________________________________________________arrow_forward1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids? 2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following: Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng Final recovery volume: 0.50 mL Volume plated: 0.25 mL Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forward
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