EBK BIOLOGY
EBK BIOLOGY
4th Edition
ISBN: 8220102797376
Author: BROOKER
Publisher: YUZU
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Chapter 10.2, Problem 1EQ
Summary Introduction

To determine: The purpose of the study conducted by Loewenstein and colleagues.

Introduction: In a multicellular organism, cells are linked to each other by specialized structures called cell junctions. Animal cells have a more varied group of cell junctions than plant cells, as they lack a cell wall. Three types of junctions found between cells in animals are anchoring junctions, tight junctions and gap junctions.

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Describe experiments using marker molecules that have been done to elucidate important properties of (1) tight junctions and (2) gap junctions.
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The following image is based on real results from an influential experiment in cell biology.  This experiment studied the role of the proteins Ran and importin in nuclear protein transport.  The experiment used flourescently-labeled proteins containing a nuclear localization sequence (the "substrate").  Ran, importin, and GTP were either present or absent, as indicated in the figure.  Cells were exposed to these different conditions and then analyzed by fluorescence microscopy. Circles are the cells' nuclei.  Very light circles are the nuclei without the substrate attached. Study the figure below and then answer the questions that follow. 1. What actions does Ran perform by itself? 2. What actions does importin perform by itself? 3. How do Ran and importin act together in the nuclear import of protein? 4. Why is GTP required?
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