Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 10, Problem 6P
Interpretation Introduction

Interpretation:

The meaning of the biochemical rationale for ATP serving as a positive regulator of ATCase is to be stated.

Concept introduction:

Enzymes being the catalysts increase the rate of reaction. In an enzyme-catalyzed reaction, the rate of reaction for the product formation depends on the concentration of enzyme as well as the substrate involved in the reaction via formation of an enzyme substrate complex.The enzyme which is able to do the conformational changes if an effector gets attached to it is known as allosteric enzyme.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)
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