Campbell Biology: Custom Edition
Campbell Biology: Custom Edition
18th Edition
ISBN: 9781323717271
Author: Urry, Cain, Wasserman, Minorsky, Reece
Publisher: PEARSON C
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Chapter 10, Problem 5TYU
Summary Introduction

Introduction: Photosynthesis is the process in which the chloroplast of plants capture sunlight, and converts it into chemical energy stored as sugar and other molecules. This occurs in two stages known as the light reactions (require light) and, the Calvin cycle (synthesis). During light reactions, water is split to give off oxygen, and produces ATP and NADPH. During Calvin cycle, a carbon dioxide molecule is accepted by a carbon acceptor molecule (ribulose-1, 5-bisphosphate, RuBP) and forms a six-carbon compound. This is converted into three-carbon sugars (glyceraldehyde-3-phosphate, G3P) through the oxidation of NADPH. Next, some of the G3P molecules form sugars and others regenerate to RuBP molecule.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.

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Photosynthesis & Respiration | Reactions | Chemistry | FuseSchool; Author: FuseSchool - Global Education;https://www.youtube.com/watch?v=3XIyweZg6Sw;License: Standard YouTube License, CC-BY