Biology
12th Edition
ISBN: 9780078024269
Author: Sylvia Mader, Michael Windelspecht
Publisher: McGraw-Hill Education
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Chapter 10, Problem 17A
Summary Introduction
Introduction:
Changes in the structure of the chromosome are another type of “chromosomal mutation”. Various agents that are present in the environment such as radiations, viruses or even few organic chemicals can cause chromosomes to break. Ordinarily when chromosome breaks occur the broken ends reunite to form same sequence of genes. However, sometimes the broken ends do not reunite in the same pattern resulting in various types of “chromosomal mutations”.
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a) What is the function of the disulfide bonds that form within and between the alpha and beta chains?
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a) The amino acid sequence of the alpha chain terminates with a *. What does this symbol mean?
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 10 Solutions
Biology
Ch. 10.1 - Prob. 1CYPCh. 10.1 - Prob. 2CYPCh. 10.1 - Prob. 3CYPCh. 10.2 - Prob. 1CYPCh. 10.2 - Prob. 2CYPCh. 10.2 - Prob. 3CYPCh. 10.3 - Prob. 1CYPCh. 10.3 - Explain what would cause daughter ceils following...Ch. 10.3 - Prob. 3CYPCh. 10.4 - Prob. 1CYP
Ch. 10.4 - 2. Explain how meiosis II is more similar to...Ch. 10.5 - Describe where cells that undergo meiosis are...Ch. 10.5 - Prob. 2CYPCh. 10.6 - 1. Explain the kinds of changes in chromosome...Ch. 10.6 - Prob. 2CYPCh. 10.6 - Prob. 3CYPCh. 10 - Prob. 1NS.1QCCh. 10 - Prob. 1NS.2QCCh. 10 - Prob. 1ACh. 10 - Prob. 2ACh. 10 - Prob. 3ACh. 10 - Prob. 4ACh. 10 - Prob. 5ACh. 10 - Prob. 6ACh. 10 - Prob. 7ACh. 10 - Prob. 8ACh. 10 - Prob. 9ACh. 10 - Prob. 10ACh. 10 - Prob. 11ACh. 10 - Prob. 12ACh. 10 - Prob. 13ACh. 10 - Prob. 14ACh. 10 - Prob. 15ACh. 10 - Prob. 16ACh. 10 - Prob. 17ACh. 10 - Prob. 18ACh. 10 - Prob. 1TSCh. 10 - Prob. 2TSCh. 10 - Prob. 3TSCh. 10 - Prob. 4TS
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- a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates? b) Predict the growth you would expect to see on each of the following plates: – DNA ___________________________________________________________ – DNA/+Amp ______________________________________________________ +DNA/+Amp ______________________________________________________ +DNA/+Amp/+IPTG _________________________________________________arrow_forward1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids? 2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following: Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng Final recovery volume: 0.50 mL Volume plated: 0.25 mL Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forward
- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forwardIn oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donorarrow_forward
- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have shipped your samples off for sequencing and are now waiting for the results. Out of curiosity (and maybe boredom...) you decide to test your culture for the Catalase and Oxidase enzymes. Upon testing your sample for catalase, you don't see any bubbles; however, you do see a color change to purple during the Oxidase test. What results can you conclude from this? O Catalase-/ Oxidase + O Catalase +/ Oxidase + Catalase + / Oxidase- O Catalase / Oxidase - O None of the abovearrow_forwardWhich of the following is not a strength of using 16S rRNA for phylogenetic analyses? OA. It's cheap OB. It's easy to do C. It can be used to identify all the way down to the strain level OD. Both A & B OE. None of the abovearrow_forwardWhy are molecular approaches important to the field of microbial taxonomy and phylogeny? Phylogenetic inferences based on molecular approaches provide the most robust analysis of microbial evolution currently available. It allows for the collection of a large and accurate dataset from many organisms Almost no fossil record was left by microbes when compared to plants and animals All of the above None of the abovearrow_forward
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