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Pearson eText Genetic Analysis: An Integrated Approach -- Instant Access (Pearson+)
3rd Edition
ISBN: 9780135564172
Author: Mark Sanders, John Bowman
Publisher: PEARSON+
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Chapter 1, Problem 25P
Summary Introduction
To review:
The size of a DNA fragment affects its electrophoretic mobility on the electrophoresis gels.
Introduction:
Electrophoretic mobility is the migration of charged particles under the influence of applied electrical field. The cations move toward the negatively charged cathode, anions move toward the positively charged anode, and neutral species remain stationary.
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A piece of DNA is cut into four fragments as shown below. A solution containing the four fragments is placed in a single well at the top of an agarose gel. Using the information given below, draw (below the well) how you think the fragments will be aligned on the gel following electrophoresis. Label each fragment with its corresponding letter. Remember, each band on the gel will be the same width, equal to the width of the well at the top of the gel. These should all be in one lane.
What is it about the chemistry of DNA that causes it to be uniformly negatively charged?
A piece of DNA is cut into four fragments as shown below. A solution containing the four fragments is placed in a single well at the top of an agarose gel. Using the information given below, draw (below the well) how you think the fragments will be aligned on the gel following electrophoresis. Label each fragment with its corresponding letter. Remember, each band on the gel will be the same width, equal to the width of the well at the top of the gel. These should all be in one lane.
What if you had two different DNA fragments that were exactly the same length as measured in base-pairs. Would it be possible to distinguish them using this type of electrophoresis? How would they appear on a gel?
Gel electrophoresis drives DNA along an electrical current from a negative electrode to a positive electrode. This is because DNA is a negatively charged molecule. Do you think that gel electrophoresis would function differently if DNA was positively charged? How so? Why?
Chapter 1 Solutions
Pearson eText Genetic Analysis: An Integrated Approach -- Instant Access (Pearson+)
Ch. 1 - 1. Genetics affects many aspects of our lives....Ch. 1 - 2. How do you think the determination that DNA is...Ch. 1 - 3. A commentator once described genetics as “the...Ch. 1 - All life shares DNA as the hereditary material....Ch. 1 - Define the terms allele, chromosome, and gene and...Ch. 1 - 6. Define the terms genotype and phenotype, and...Ch. 1 - 7. Define natural selection, and describe how...Ch. 1 - Describe the modern synthesis of evolution, and...Ch. 1 - What are the four processes of evolution? Briefly...Ch. 1 - Define each of the following terms: a....
Ch. 1 - 11. Compare and contrast the genome, the proteome,...Ch. 1 - With respect to transcription describe the...Ch. 1 - Plant agriculture and animal domestication...Ch. 1 - Briefly describe the contribution each of the...Ch. 1 - If thymine makes up 21% of the DNA nucleotides in...Ch. 1 - What reactive chemical groups are found at the 5...Ch. 1 - Identify two differences in chemical composition...Ch. 1 - What is the central dogma of molecular biology?...Ch. 1 - A portion of a polypeptide contains the amino...Ch. 1 - The following segment of DNA is the template...Ch. 1 - 23. Fill in the missing nucleotides (so there are...Ch. 1 - 24. Suppose a genotype for a protein-producing...Ch. 1 - Prob. 25PCh. 1 - 26. Four nucleic acid samples are analyzed to...Ch. 1 - 27. What is meant by the term homology? How is...Ch. 1 - 28. If one is constructing a phylogeny of reptiles...Ch. 1 - 29. Consider the following segment of...Ch. 1 - 30. Ethical and social issues have become a large...Ch. 1 - 31. In certain cases, genetic testing can identify...Ch. 1 - 32. What information presented in this chapter and...Ch. 1 - 33. It is common to study the biology and genetics...
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- Calculate the length of the 50,000-bp DNA in a 30-nm fiber.arrow_forwardYou study actin polymerization by monitoring the increase in fluorescence of pyrene-labeled actin. Pyrene is a fluorescent molecule, which has very low level of fluorescence on its own or when fused to G-actin. However, the pyrene fluorescence increases dramatically when pyrene-labeled G-actin is incorporated into a polymer. In this assay, the amount of pyrene fluorescence is proportional to the amount of actin polymer. In the figure below, actin polymerization curves resulted for conditions listed below; assume that G-actin concentration is above the critical concentration for the pointed end and that ATP is present. Which curve corresponds to which condition? Explainarrow_forwardGive the purpose of gel electrophoresis system. If larger DNA is loaded in the gel, what will be the movement of such DNA, how about is smaller DNA is loaded into the gel?arrow_forward
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- Give typing answer with explanation and conclusionarrow_forwardExplain why two metal ions play important role on the palm domain of DNA polymerase.arrow_forwardWhich of the following statements are true regarding the properties of DNA and RNA polymerase. Select all that apply. Both DNA and RNA polymerase synthesize nucleic acid strands in the 5" to 3' direction. Both DNA and RNA polymerase can initiate strand synthesis on their own. I. RNA polymerase initiates strand synthesis, while DNA polymerase depends upon an existing strand to continue synthesis. II. RNA polymerase only uses ribonucleotides for strand synthesis. DNA polymerase only uses deoxyribonucleotides for strand synthesis. V. Au DNA and RNA polymerases from eukaryotes behave very differently from DNA and RNA polymerases found in prokaryotes. O VI.arrow_forward
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