Microbiology: Principles and Explorations
10th Edition
ISBN: 9781119390114
Author: Black
Publisher: WILEY
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Textbook Question
Chapter 1, Problem 2.4SC
Why was the French microbiologists’ method of broth dilution inadequate for obtaining pure cultures of organisms?
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Chapter 1 Solutions
Microbiology: Principles and Explorations
Ch. 1 - List three reasons to study microbiology.Ch. 1 - What is the difference between microbiology and...Ch. 1 - Prob. 1.3SCCh. 1 - List five bacterial diseases and five viral...Ch. 1 - Prob. 2.1SCCh. 1 - State the germ theory of disease. Try to think of...Ch. 1 - How did Pasteurs experiment with swan-necked...Ch. 1 - Why was the French microbiologists method of broth...Ch. 1 - What were the scientific contributions of Jenner,...Ch. 1 - Prob. 3.2SC
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What are the advantages and disadvantages of culture-dependent and culture-independent methods in microbiology, respectively.arrow_forwardWhy might viable cell cultures be of more use in microbialtaxonomy than preserved specimens?arrow_forwardWhat is the importance of using a test/control organism in describing the cultural characteristics of an unknown bacterium?arrow_forward
- What is the purpose of the confirmed test in an experiment designed to test for coliform bacteria?arrow_forwardThe use of sterile techniques and agar media enables microbiologists to study: a) Bacterial growth b) Nutrient agar-metabolizing culture c) Bacterial metabolism d) Pure cultures d) Bacterial life cyclarrow_forwardWhat is the goal of isolation streak plate technique? Why is microbial considered a pure culture?arrow_forward
- 1. A plate with a final dilution factor of 107 produced 210 colonies. a) What was the original concentration in the sample? b) If you were to dilute the original sample with a dilution factor of 108, how many colonies would you count on the plate? c) If you were to dilute the original sample with a dilution factor of 106, how many colonies would you count on the plate? 2. Sally Monella found 344 bacterial colonies on one of her plates. She had prepared this plate after a serial dilution of a culture of Yersinia pestis. She had pipetted 1 ml sample of diluted Yersinia pestis from a tube with the overall dilution factor of 106 into a plate and covered it with agar. She used this plate to calculate the concentration in cells/ml of a bacterial culture. a) How many organisms were in the original culture? b) Were her results valid? Why or why not?arrow_forwardA student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step what was done to accomplish this. The transfer of bacteria was not successful because of which step? 1. Cap of the broth culture is removed 2. The mouth of the bottle is flamed 3. The loop was flamed 4. The loop was inserted into the culture to pick up the bacteria 5. The loop was flamed 6. The loop containing the bacteria was used to introduced to spread the agar plate 7. The plate was placed in an incubator at 30 Celciusarrow_forwardYou mixed up the numbers on the tubes when you inoculated the mannitol salt agar (MSA) plate. You do not know if you grew staph epidermis or E. coli. You found that the organism growing on the mannitol salt agar remained red after incubation. It is most likely that the organism is E.coli. a) True b) Falsearrow_forward
- What are the advantages and disadvantages of plate count technique over other methods of quantifying microbial populations?arrow_forwardWhat would happen if the enrichment method in the isolation of bacteriophage was omitted?the chloroform was not added to the enrichment?the 0.1 ml lysate - E.coli mix was plated directly on top of the bottom agar?arrow_forwardYou inoculated a culture with an initial cell count of 6.5x10^3 cells. The generation time for this organism is 25 minutes. You grew the culture for 10 hours. a) How many generations occurred?b) How many cells will be present after the 10 hours?arrow_forward
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