Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 1, Problem 1CT
Summary Introduction
To determine:
The number of cells of Bacillus and yeast can be observed under a field of 2mm diameter.
Introduction:
Microscopy is a technique of observing an object under microscope that is not observable with naked eyes. The types of microscopy include, scanning probe microscopy, electron microscopy and optical microscopy. Optical microscopy uses light for illuminating and lenses to magnify the sample.
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If the diameter of the field of view in your microscope is 2 mm under low power, and one Bacillus
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Chapter 1 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
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- In the cell wall staining experiment of Bacillus megaterium, what color can you find in a) cell wall and why? b) cytoplasm and why?arrow_forwardUsing bright-field microscopy to look at a slide prepared with a basic dye you observe cells with a clear inner compartment within the cell at 400X magnification. The cell is most likely a(n) A) prokaryote. B) bacterium. C) archaeon. D) eukaryote.arrow_forwardAssume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope. c) After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred Provide a description of the procedure to prepare an acidic stain of bacteria using Nigrosin as it would appear in the Methods section.arrow_forward
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