BIOCHEMISTRY (LOOSELEAF) >CUSTOM PKG<
BIOCHEMISTRY (LOOSELEAF) >CUSTOM PKG<
8th Edition
ISBN: 9781305760738
Author: Campbell
Publisher: CENGAGE C
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Chapter 1, Problem 11RE

REFLECT AND APPLY Why is the development of catalysis important to the development of life?

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Example 1: 1. Suppose an enzyme (MW = 5,000 g/mole) has a concentration of 0.05 mg/L. If the kcat is 1 x 10 s, what is the theoretical maximum reaction velocity for the enzyme? A) 1050 µM/s. B) 100 µM/s. C) 150 μM/s. D) 105 μM/s.
In 1956, E. P. Kennedy and S. B. Weiss published their study of membrane lipid phosphatidylcholine (lecithin) synthesis in rat liver. Their hypothesis was that phosphocholine joined with some cellular component to yield lecithin. In an earlier experiment, incubating 32 P-labeled phosphocholine at physiological temperature (37 °C) with broken cells from rat liver yielded labeled lecithin. This became their assay for the enzymes involved in lecithin synthesis. Determine the optimal pH for this enzyme and characterize the enzyme activity at different pH values. -O-P-O-CH2-CH₁₂-N(CH3)3 Phosphocholine H₂C-O-C-R HC-O-C-R2 + + + Cell fraction + ? HC-O-P-O-CH₁₂-CH₂-N(CH), O Phosphatidylcholine The researchers then centrifuged the broken cell preparation to separate the membranes from the soluble proteins. They tested three preparations: whole extract, membranes, and soluble proteins. Table 1 summarizes the results. Table 1: Cell fraction requirement for incorporation of 32p-phosphocholine into…
Researchers isolated an unknown substance, X, from rabbit muscle. They determined its structure from the following observations and experiments. (a) Qualitative analysis showed that X was composed entirely of C, H, and O. A weighed sample of X was completely oxidized and the H2O and CO2 produced were measured. This quantitative analysis revealed that X contained 40.00% C, 6.71% H, and 53.29% O by weight. (b) The molecular mass of X, as determined by mass spectrometry, was 90.00 atomic mass units (u). (c) Infrared spectroscopy showed that X contained one double bond. (d) X dissolved readily in water, and the solution demonstrated optical activity when tested in a polarimeter. (e) The aqueous solution of X is acidic. What is the empirical formula of X?

Chapter 1 Solutions

BIOCHEMISTRY (LOOSELEAF) >CUSTOM PKG<

Ch. 1 - REFLECT AND APPLY Why is the development of...Ch. 1 - REFLECT AND APPLY What are two major advantages of...Ch. 1 - REFLECT AND APPLY Why was the development of a...Ch. 1 - REFLECT AND APPLY Comment on RNAs role in...Ch. 1 - REFLECT AND APPLY Do you consider it a reasonable...Ch. 1 - RECALL List five differences between prokaryotes...Ch. 1 - RECALL Do the sites of protein synthesis differ in...Ch. 1 - REFLECT AND APPLY Assume that a scientist claims...Ch. 1 - RECALL Draw an idealized animal cell, and identify...Ch. 1 - RECALL Draw an idealized plant cell, and identify...Ch. 1 - RECALL What are the differences between the...Ch. 1 - RECALL Which organelles are surrounded by a double...Ch. 1 - RECALL Which organelles contain DNA?Ch. 1 - RECALL Which organelles are the sites of...Ch. 1 - RECALL State how the following organelles differ...Ch. 1 - RECALL List the five kingdoms into which living...Ch. 1 - RECALL Which of the five kingdoms consist of...Ch. 1 - RECALL How does the five-kingdom classification...Ch. 1 - REFLECT AND APPLY What are the advantages of being...Ch. 1 - REFLECT AND APPLY Mitochondria and chloroplasts...Ch. 1 - REFLECT AND APPLY Fossil evidence indicates that...Ch. 1 - RECALL Which processes are favored: those that...Ch. 1 - RECALL Does the thermodynamic term spontaneous...Ch. 1 - BIOCHEMICAL CONNECTIONS For the process...Ch. 1 - RECALL Which of the following are spontaneous...Ch. 1 - REFLECT AND APPLY In which of the following...Ch. 1 - REFLECT AND APPLY Why is it necessary to specify...Ch. 1 - REFLECT AND APPLY Why is the entropy of a system...Ch. 1 - REFLECT AND APPLY A reaction at 23C has G=1kJmol1....Ch. 1 - REFLECT AND APPLY Urea dissolves very readily in...Ch. 1 - REFLECT AND APPLY Would you expect the reaction...Ch. 1 - REFLECT AND APPLY The existence of organelles in...Ch. 1 - REFLECT AND APPLY Why is it advantageous for a...Ch. 1 - REFLECT AND APPLY Which would you expect to have a...Ch. 1 - REFLECT AND APPLY How would you modify your answer...Ch. 1 - REFLECT AND APPLY Would it be more or less likely...Ch. 1 - REFLECT AND APPLY What thermodynamic...Ch. 1 - REFLECT AND APPLY If cells of the kind we know...Ch. 1 - REFLECT AND APPLY The process of protein folding...Ch. 1 - REFLECT AND APPLY In biochemistry, the exergonic...
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