You're an undercover biological weapons agent who has been given an unknown liquid - you suspect that it is just sugar water (because you drank some by accident - you know you're not supposed to have food or drink in the lab, but you do it all the time, and it tasted sweet - plus you didn't die!) One of the tests you need to run is to see if it contains protein (remember, foreign proteins can cause severe immune responses which can be a powerful biological weapon). You set up 3 test tubes each with 10mL of the following: distilled water, gelatin solution and the unknown. The distilled water is clear, the gelatin solution is clear with a beige tint, and the unknown is clear. You use Biuret's Reagent - a dark blue dye which will turn a liquid sample purple in the presence of protein. 1. When Biuret's Reagen is added to the unknown, predict what color you expect it to become if the unknown is just sugar water: [a] (no change | blue | purple). 2.You included both a positive and negative control for this experiment. The water is the [c] control. 3.If the positive control turns blue, it means: (select all that applies) there is no protein present this control is no good - need to troubleshoot and perform the experiment again there is protein present this control is doing exactly what its supposed to do - you can have confidence that the results of the unknown will be accurate
You're an undercover biological weapons agent who has been given an unknown liquid - you suspect that it is just sugar water (because you drank some by accident - you know you're not supposed to have food or drink in the lab, but you do it all the time, and it tasted sweet - plus you didn't die!) One of the tests you need to run is to see if it contains protein (remember, foreign proteins can cause severe immune responses which can be a powerful biological weapon). You set up 3 test tubes each with 10mL of the following: distilled water, gelatin solution and the unknown.
The distilled water is clear, the gelatin solution is clear with a beige tint, and the unknown is clear.
You use Biuret's Reagent - a dark blue dye which will turn a liquid sample purple in the presence of protein.
1. When Biuret's Reagen is added to the unknown, predict what color you expect it to become if the unknown is just sugar water: [a] (no change | blue | purple).
2.You included both a positive and negative control for this experiment. The water is the [c] control.
3.If the positive control turns blue, it means: (select all that applies)
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there is no protein present |
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this control is no good - need to troubleshoot and perform the experiment again |
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there is protein present |
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this control is doing exactly what its supposed to do - you can have confidence that the results of the unknown will be accurate |
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