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You wish to separate two proteins, A and B, using precipitation with ammonium sulfate. Protein A has a solubility of 12.2 g/L in 1 M salt and 164 g/L in pure water. Protein B has a solubility of 4.1 g/L in 2 M salt and 0.41 g/L in 3 M salt. If you have a protein solution with 20 g/L A and 5 g/L B and you desire a precipitate that is pure A, what is the maximum percentage recovery of A by precipitation? What is the composition of the remaining solution (referred to as the “mother liquor”) after precipitation?

You wish to separate two proteins, A and B, using precipitation with ammonium sulfate. Protein A has a solubility of 12.2 g/L in 1 M salt and 164 g/L in pure water. Protein B has a solubility of 4.1 g/L in 2 M salt and 0.41 g/L in 3 M salt. If you have a protein solution with 20 g/L A and 5 g/L B and you desire a precipitate that is pure A, what is the maximum percentage recovery of A by precipitation? What is the composition of the remaining solution (referred to as the “mother liquor”) after precipitation?

Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
Problem 1.1P
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You wish to separate two proteins, A and B, using precipitation with ammonium sulfate. Protein A has a solubility of 12.2 g/L in 1 M salt and 164 g/L in pure water. Protein B has a solubility of 4.1 g/L in 2 M salt and 0.41 g/L in 3 M salt.

If you have a protein solution with 20 g/L A and 5 g/L B and you desire a precipitate that is pure A, what is the maximum percentage recovery of A by precipitation? What is the composition of the remaining solution (referred to as the “mother liquor”) after precipitation?

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