You need to prepare 500 ml PBS buffer, a solution commonly used in biological research. The buffer helps to maintain the pH, and the osmolarity, as well as ion concentrations of PBS buffer, match those of the human body. PBS buffer consists of the following components: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4. How would you prepare the buffer?
You need to prepare 500 ml PBS buffer, a solution commonly used in biological research. The buffer helps to maintain the pH, and the osmolarity, as well as ion concentrations of PBS buffer, match those of the human body. PBS buffer consists of the following components: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4. How would you prepare the buffer?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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You need to prepare 500 ml PBS buffer, a solution commonly used in biological research. The buffer helps to maintain the pH, and the osmolarity, as well as ion concentrations of PBS buffer, match those of the human body. PBS buffer consists of the following components: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4. How would you prepare the buffer?
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