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You have a patient with a sore throat. You suspect Streptococcus pyogenes. S. pyogenes is a Gram-positive cocci that is beta hemolytic. It is not a halophile. It does not ferment lactose or mannitol. What would you observe if you plated this sample on a SBA plate?

You have a patient with a sore throat. You suspect Streptococcus pyogenes. S. pyogenes is a Gram-positive cocci that is beta hemolytic. It is not a halophile. It does not ferment lactose or mannitol. What would you observe if you plated this sample on a SBA plate?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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**Case Study: Suspected Streptococcus pyogenes Infection** You have a patient with a sore throat. You suspect *Streptococcus pyogenes*. *S. pyogenes* is a Gram-positive cocci that is beta hemolytic. It is not a halophile. It does not ferment lactose or mannitol. What would you observe if you plated this sample on a SBA (Sheep Blood Agar) plate? **Image Descriptions and Analysis:** 1. **First Image**: Shows a streak of bacterial growth with a clear zone surrounding it on a red agar plate. - **Explanation**: This demonstrates beta hemolysis, where the bacteria completely lyse the red blood cells in the agar, creating a clear zone around the colonies. This pattern is characteristic of *Streptococcus pyogenes*. 2. **Second Image**: Displays large, greyish-white colonies that are not hemolytic. - **Explanation**: This represents non-hemolytic bacteria, which is inconsistent with *S. pyogenes*, as it should exhibit clear beta hemolysis. 3. **Third Image**: Shows several isolated colonies on a red agar surface, with no clear zone visible. - **Explanation**: This demonstrates gamma hemolysis, where there is no lysis of red blood cells, thus not indicative of *S. pyogenes*. 4. **Fourth Image**: A completely red agar plate with no observable growth or clear zones. - **Explanation**: This indicates no bacterial growth, or possibly alpha hemolysis, where only partial hemolysis occurs. This is not representative of *S. pyogenes*. 5. **Fifth Image**: Displays a red agar background with a dark, undefined form. - **Explanation**: This is unclear without further details, but does not distinctly show beta hemolysis. **Conclusion:** For *Streptococcus pyogenes*, the expected observation on a SBA plate is beta hemolysis, as depicted in the first image. This manifestation is characterized by the clear zones surrounding the colonies, confirming the lysis of red blood cells.
Transcribed Image Text:**Case Study: Suspected Streptococcus pyogenes Infection** You have a patient with a sore throat. You suspect *Streptococcus pyogenes*. *S. pyogenes* is a Gram-positive cocci that is beta hemolytic. It is not a halophile. It does not ferment lactose or mannitol. What would you observe if you plated this sample on a SBA (Sheep Blood Agar) plate? **Image Descriptions and Analysis:** 1. **First Image**: Shows a streak of bacterial growth with a clear zone surrounding it on a red agar plate. - **Explanation**: This demonstrates beta hemolysis, where the bacteria completely lyse the red blood cells in the agar, creating a clear zone around the colonies. This pattern is characteristic of *Streptococcus pyogenes*. 2. **Second Image**: Displays large, greyish-white colonies that are not hemolytic. - **Explanation**: This represents non-hemolytic bacteria, which is inconsistent with *S. pyogenes*, as it should exhibit clear beta hemolysis. 3. **Third Image**: Shows several isolated colonies on a red agar surface, with no clear zone visible. - **Explanation**: This demonstrates gamma hemolysis, where there is no lysis of red blood cells, thus not indicative of *S. pyogenes*. 4. **Fourth Image**: A completely red agar plate with no observable growth or clear zones. - **Explanation**: This indicates no bacterial growth, or possibly alpha hemolysis, where only partial hemolysis occurs. This is not representative of *S. pyogenes*. 5. **Fifth Image**: Displays a red agar background with a dark, undefined form. - **Explanation**: This is unclear without further details, but does not distinctly show beta hemolysis. **Conclusion:** For *Streptococcus pyogenes*, the expected observation on a SBA plate is beta hemolysis, as depicted in the first image. This manifestation is characterized by the clear zones surrounding the colonies, confirming the lysis of red blood cells.
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