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You have a 25 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 7.93. You incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. At the end of the incubation period, you measure the pH again and find that it has decreased to 6.15. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in the original 25 mL sample. Remember to use significant figures in your answer. CH, CH, сH, —с —о-сн,— сн, — N—сн, H,0 но-сн, —сн,—"N—CH, + сн,—с —о- + н CH, ČH, Acetylcholine Choline Acetate

You have a 25 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 7.93. You incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. At the end of the incubation period, you measure the pH again and find that it has decreased to 6.15. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in the original 25 mL sample. Remember to use significant figures in your answer. CH, CH, сH, —с —о-сн,— сн, — N—сн, H,0 но-сн, —сн,—"N—CH, + сн,—с —о- + н CH, ČH, Acetylcholine Choline Acetate

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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You have a 25 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 7.93. You incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. At the end of the incubation period, you measure the pH again and find that it has decreased to 6.15. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in the original 25 mL sample. Remember to use significant figures in your answer. сн, CH, сH, —с —о—сн, — сн, — N—сн, H,0 но—сн, ——сн,— "N—сн, + сH,—ҫ—о + H CH, ČH, Acetylcholine Choline Acetate 7.02 acetycholine in original sample: nmol Incorrect
Transcribed Image Text:You have a 25 mL sample of acetylcholine (a neurotransmitter) with an unknown concentration and a pH of 7.93. You incubate this sample with the enzyme acetylcholinesterase to convert all of the acetylcholine to choline and acetic acid. The acetic acid dissociates to yield acetate and hydrogen ions. At the end of the incubation period, you measure the pH again and find that it has decreased to 6.15. Assuming there was no buffer in the assay mixture, determine the number of nanomoles of acetylcholine in the original 25 mL sample. Remember to use significant figures in your answer. сн, CH, сH, —с —о—сн, — сн, — N—сн, H,0 но—сн, ——сн,— "N—сн, + сH,—ҫ—о + H CH, ČH, Acetylcholine Choline Acetate 7.02 acetycholine in original sample: nmol Incorrect
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