You do a viral plaque assay using the six well plate shown below. The dilution scheme, what was infected on each well, and the results of the plaque assay are shown below. What is the viral titer of the original lysate?  A. ~1.1 *10^6 PFU/mL   B. ~1 *10^7 PFU/mL   C. ~3 * 10^7 PFU/mL   D. ~2 * 10^6 PFU/mL   E. ~3.5 *10^6 PFU/mL   F. ~1.1 * 10^5 PFU/mL   G. ~3.5 *10^5 PFU/mL

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You do a viral plaque assay using the six well plate shown below. The dilution scheme, what was infected on each well, and the results of the plaque assay are shown below. What is the viral titer of the original lysate? 

A.

~1.1 *10^6 PFU/mL

  B.

~1 *10^7 PFU/mL

  C.

~3 * 10^7 PFU/mL

  D.

~2 * 10^6 PFU/mL

  E.

~3.5 *10^6 PFU/mL

  F.

~1.1 * 10^5 PFU/mL

  G.

~3.5 *10^5 PFU/mL

30 ul
300ul
300ul
300ul
300ul
Pure Viral
Lysate
2.97 mL
media
2.7mL
media
2.7 mL
2.7mL
media
Infect well Infect well
2.7mL
media
media
Infect well
A2 with
Infect well
A3 with
Infect well
83 with
100 ul
B1 with
B2 with
100 ul
100 ul
100 ul
100 ul
2.
Transcribed Image Text:30 ul 300ul 300ul 300ul 300ul Pure Viral Lysate 2.97 mL media 2.7mL media 2.7 mL 2.7mL media Infect well Infect well 2.7mL media media Infect well A2 with Infect well A3 with Infect well 83 with 100 ul B1 with B2 with 100 ul 100 ul 100 ul 100 ul 2.
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