You do a viral plaque assay using the six well plate shown below. The dilution scheme, what was infected on each well, and the results of the plaque assay are shown below. What is the viral titer of the original lysate? A. ~1.1 *10^6 PFU/mL B. ~1 *10^7 PFU/mL C. ~3 * 10^7 PFU/mL D. ~2 * 10^6 PFU/mL E. ~3.5 *10^6 PFU/mL F. ~1.1 * 10^5 PFU/mL G. ~3.5 *10^5 PFU/mL
You do a viral plaque assay using the six well plate shown below. The dilution scheme, what was infected on each well, and the results of the plaque assay are shown below. What is the viral titer of the original lysate? A. ~1.1 *10^6 PFU/mL B. ~1 *10^7 PFU/mL C. ~3 * 10^7 PFU/mL D. ~2 * 10^6 PFU/mL E. ~3.5 *10^6 PFU/mL F. ~1.1 * 10^5 PFU/mL G. ~3.5 *10^5 PFU/mL
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question
You do a viral plaque assay using the six well plate shown below. The dilution scheme, what was infected on each well, and the results of the plaque assay are shown below. What is the viral titer of the original lysate?
A. |
~1.1 *10^6 PFU/mL |
||
B. |
~1 *10^7 PFU/mL |
||
C. |
~3 * 10^7 PFU/mL |
||
D. |
|
||
E. |
~3.5 *10^6 PFU/mL |
||
F. |
~1.1 * 10^5 PFU/mL |
||
G. |
~3.5 *10^5 PFU/mL |
![30 ul
300ul
300ul
300ul
300ul
Pure Viral
Lysate
2.97 mL
media
2.7mL
media
2.7 mL
2.7mL
media
Infect well Infect well
2.7mL
media
media
Infect well
A2 with
Infect well
A3 with
Infect well
83 with
100 ul
B1 with
B2 with
100 ul
100 ul
100 ul
100 ul
2.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F1268647a-dc9a-4138-9f31-b5c0771d2ded%2Fac319147-bdb8-42c1-b321-0d34edb14c66%2Fhzamk9q_processed.jpeg&w=3840&q=75)
Transcribed Image Text:30 ul
300ul
300ul
300ul
300ul
Pure Viral
Lysate
2.97 mL
media
2.7mL
media
2.7 mL
2.7mL
media
Infect well Infect well
2.7mL
media
media
Infect well
A2 with
Infect well
A3 with
Infect well
83 with
100 ul
B1 with
B2 with
100 ul
100 ul
100 ul
100 ul
2.
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