You design a protein with an N-terminal signal sequence and a single transmembrane domain. You also decide to tag your protein with GFP. If you want the GFP to be outside the cell, where should the GFP appear in the amino acid sequence? a. Between the transmembrane domain and the C-terminus b. Between the signal sequence and the transmembrane domain c. The GFP can be anywhere in the amino acid sequence d. At the C-terminal end of the protein (i.e., the last part of the protein to be translated
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
You design a protein with an N-terminal signal sequence and a single transmembrane domain. You also decide to tag your protein with GFP. If you want the GFP to be outside the cell, where should the GFP appear in the amino acid sequence?
Between the transmembrane domain and the C-terminus
Between the signal sequence and the transmembrane domain
The GFP can be anywhere in the amino acid sequence
At the C-terminal end of the protein (i.e., the last part of the protein to be translated
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